ArticleNature protocols2012
Fusion of single proteoliposomes with planar, cushioned bilayers in microfluidic flow cells.
Article in Nature protocols, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed.
- Munc18 modulates syntaxin phase separation to promote exocytosis.Nature neuroscience · 2026Article
- Synergistic regulation of fusion pore opening and dilation by SNARE and synaptotagmin-1.Journal of molecular cell biology · 2024Article
- Dynamics of membrane tubulation coupled with fission by a two-component module.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Size-Based Sorting and In Situ Clonal Expansion of Single Cells Using Microfluidics.Biosensors · 2022Article
- Structural and biochemical insights into lipid transport by VPS13 proteins.The Journal of cell biology · 2022Article
- Optimal Detection of Fusion Pore Dynamics Using Polarized Total Internal Reflection Fluorescence Microscopy.Frontiers in molecular biosciences · 2021Article
- Retromer forms low order oligomers on supported lipid bilayers.The Journal of biological chemistry · 2020Article
- Fusion Pore Formation Observed during SNARE-Mediated Vesicle Fusion with Pore-Spanning Membranes.Biophysical journal · 2020Article
- A micropatterned substrate for on-surface enzymatic labelling of linearized long DNA molecules.Scientific reports · 2019Article
- Highly integrated microfluidic device for cell pairing, fusion and culture.Biomicrofluidics · 2019Article
- Unraveling the mechanisms of calcium-dependent secretion.The Journal of general physiology · 2019Review
- A Nanodisc-Cell Fusion Assay with Single-Pore Sensitivity and Sub-millisecond Time Resolution.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Article
- Productive and Non-productive Pathways for Synaptotagmin 1 to Support CaFrontiers in molecular neuroscience · 2017Article
- SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy.Journal of visualized experiments : JoVE · 2016Article
- Nanodisc-cell fusion: control of fusion pore nucleation and lifetimes by SNARE protein transmembrane domains.Scientific reports · 2016Article
- Cholesterol Increases the Openness of SNARE-Mediated Flickering Fusion Pores.Biophysical journal · 2016Article
- A Programmable DNA Origami Platform to Organize SNAREs for Membrane Fusion.Journal of the American Chemical Society · 2016Article
- Image Restoration and Analysis of Influenza Virions Binding to Membrane Receptors Reveal Adhesion-Strengthening Kinetics.PloS one · 2016Article
- Histones cause aggregation and fusion of lipid vesicles containing phosphatidylinositol-4-phosphate.Biophysical journal · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Many biological processes rely on membrane fusion, and therefore assays to study its mechanisms are necessary. Here we report an assay with sensitivity to single-vesicle, and even to single-molecule events using fluorescently labeled vesicle-associated v-SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) liposomes and target-membrane-associated t-SNARE-reconstituted planar, supported bilayers (t-SBLs). Docking and fusion events can be detected using conventional far-field epifluorescence or total internal reflection fluorescence microscopy. In this assay, fusion is dependent on SNAP-25, one of the t-SNARE subunits that is required for fusion in vivo. The success of the assay is due to the use of: (i) bilayers covered with a thin layer of poly(ethylene glycol) (PEG) to control bilayer-bilayer and bilayer-substrate interactions, and (ii) microfluidic flow channels that present many advantages, such as the removal of nonspecifically bound liposomes by flow. The protocol takes 6-8 d to complete. Analysis can take up to 2 weeks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.