ArticleChemical communications (Cambridge, England)2012
Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging.
Article in Chemical communications (Cambridge, England), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Integrated biophysical and spatial remodeling during insulin secretory granule maturation at the mitochondrial network.bioRxiv : the preprint server for biology · 2026Article
- Measuring pH in insulin secretory granules by phasor-based fluorescence lifetime imaging of a genetically encoded sensor.Communications biology · 2025Article
- FLIM-Phasor Analysis (FLIM-ϕ) of Aβ-Induced Membrane Order Alterations: Towards a Cell-Based Biosensor for Early Alzheimer's Disease Diagnosis.Micromachines · 2025Article
- Decoding Insulin Secretory Granule Maturation Using Genetically Encoded pH Sensors.ACS sensors · 2024Article
- Development of Highly Fluorogenic Styrene Probes for Visualizing RNA in Live Cells.ACS chemical biology · 2023Article
- A Single Fluorescent Protein-Based Indicator with a Time-Resolved Fluorescence Readout for Precise pH Measurements in the Alkaline Range.International journal of molecular sciences · 2022Article
- Phasor Analysis of Fluorescence Lifetime Enables Quantitative Multiplexed Molecular Imaging of Three Probes.Analytical chemistry · 2022Article
- Resolving subcellular pH with a quantitative fluorescent lifetime biosensor.Nature communications · 2022Article
- Genetically Encoded Ratiometric pH Sensors for the Measurement of Intra- and Extracellular pH and Internalization Rates.Biosensors · 2022Article
- Fluorescence lifetime-based pH mapping of tumors in vivo using genetically encoded sensor SypHerRed.Biophysical journal · 2022Article
- An EfficientInternational journal of molecular sciences · 2022Article
- Direct Photon-by-Photon Analysis of Time-Resolved Pulsed Excitation Data using Bayesian Nonparametrics.Cell reports. Physical science · 2020Article
- Carbon Dots for Intracellular pH Sensing with Fluorescence Lifetime Imaging Microscopy.Nanomaterials (Basel, Switzerland) · 2020Article
- Effects of Low Frequency-Low Voltage Alternating Electric Current on Apoptosis Progression in Bioelectrical Reactor Biofilm.Frontiers in bioengineering and biotechnology · 2020Article
- Lipid-Conjugated Rigidochromic Probe Discloses Membrane Alteration in Model Cells of Krabbe Disease.Biophysical journal · 2019Article
- Fit-free analysis of fluorescence lifetime imaging data using the phasor approach.Nature protocols · 2018Article
- Evaluating integrin activation with time-resolved flow cytometry.Journal of biomedical optics · 2018Article
- Time-Resolved Imaging Reveals Heterogeneous Landscapes of Nanomolar Ca(2+) in Neurons and Astroglia.Neuron · 2015Article
- Extended output phasor representation of multi-spectral fluorescence lifetime imaging microscopy.Biomedical optics express · 2015Article
- Induced pH-dependent shift by local surface plasmon resonance in functionalized gold nanorods.Nanoscale research letters · 2013Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
A versatile pH-dependent fluorescent protein was applied to intracellular pH measurements by means of the phasor approach to fluorescence lifetime imaging. By this fit-less method we obtain intracellular pH maps under resting or altered physiological conditions by single-photon confocal or two-photon microscopy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.