ArticleMolecular cell2012
Reconstitution of an Argonaute-dependent small RNA biogenesis pathway reveals a handover mechanism involving the RNA exosome and the exonuclease QIP.
Article in Molecular cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
- Argonaute1-Dependent LtmilR2 Negatively Regulated Infection of Lasiodiplodia theobromae by Targeting a Guanine Nucleotide Exchange Factor in RAS Signalling.Plant, cell & environment · 2025Article
- Fungal small RNA: unveiling the breakthroughs and promising applications.Frontiers in fungal biology · 2025Review
- Argonaute-independent, Dicer-dependent antiviral defense against RNA viruses.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- FoQDE2-dependent milRNA promotes Fusarium oxysporum f. sp. cubense virulence by silencing a glycosyl hydrolase coding gene expression.PLoS pathogens · 2022Article
- Nuclear and cytoplasmic RNA exosomes and PELOTA1 prevent miRNA-induced secondary siRNA production in Arabidopsis.Nucleic acids research · 2022Article
- Comparative profiling of canonical and non-canonical small RNAs in the rice blast fungus,Frontiers in microbiology · 2022Article
- Mutations in PpAGO3 Lead to Enhanced Virulence ofFrontiers in microbiology · 2022Article
- Structural and biochemical insights into small RNA 3' end trimming by Arabidopsis SDN1.Nature communications · 2018Article
- miRge 2.0 for comprehensive analysis of microRNA sequencing data.BMC bioinformatics · 2018Article
- Dicer-Like Proteins Regulate Sexual Development via the Biogenesis of Perithecium-Specific MicroRNAs in a Plant Pathogenic FungusFrontiers in microbiology · 2018Article
- Article
- Dicer-like Proteins Regulate the Growth, Conidiation, and Pathogenicity ofFrontiers in microbiology · 2017Article
- A Dicer-Independent Route for Biogenesis of siRNAs that Direct DNA Methylation in Arabidopsis.Molecular cell · 2016Article
- Article
- Intertwined pathways for Argonaute-mediated microRNA biogenesis in Drosophila.Nucleic acids research · 2014Article
- Seq and CLIP through the miRNA world.Genome biology · 2014Review
- RNAi function, diversity, and loss in the fungal kingdom.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2013Review
- Functional diversity of RNAi-associated sRNAs in fungi.International journal of molecular sciences · 2013Review
- Comprehensive investigation of microRNAs enhanced by analysis of sequence variants, expression patterns, ARGONAUTE loading, and target cleavage.Plant physiology · 2013Article
- An arrayed RNA interference genome-wide screen identifies candidate genes involved in the MicroRNA 21 biogenesis pathway.Assay and drug development technologies · 2013Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Argonaute proteins are required for the biogenesis of some small RNAs (sRNAs), including the PIWI-interacting RNAs and some microRNAs. How Argonautes mediate maturation of sRNAs independent of their slicer activity is not clear. The maturation of the Neurospora microRNA-like sRNA, milR-1, requires the Argonaute protein QDE-2, Dicer, and QIP. Here, we reconstitute this Argonaute-dependent sRNA biogenesis pathway in vitro and discover that the RNA exosome is also required for milR-1 production. Our results demonstrate that QDE-2 mediates milR-1 maturation by recruiting exosome and QIP and by determining the size of milR-1. The exonuclease QIP first separates the QDE-2-bound pre-milR-1 duplex and then mediates 3' to 5' trimming and maturation of pre-milRNA together with exosome using a handover mechanism. In addition, exosome is also important for the decay of sRNAs. Together, our results establish a biochemical mechanism of an Argonaute-dependent sRNA biogenesis pathway and critical roles of exosome in sRNA processing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.