ArticleJournal of controlled release : official journal of the Controlled Release Society2012
Microneedle delivery of plasmid DNA to living human skin: Formulation coating, skin insertion and gene expression.
Article in Journal of controlled release : official journal of the Controlled Release Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 143 citations in OpenAlex.
- A systematic review of carbohydrate-based microneedles: current status and future prospects.Journal of materials science. Materials in medicine · 2021Pooled it
- Fabrication and characterization evaluation of sustained-release dissolving microneedles with skin soothing function.Drug delivery and translational research · 2026Article
- Article
- Smart micro/nanoneedles for gene delivery.Iranian journal of basic medical sciences · 2026Review
- The next-generation DNA vaccine platforms and delivery systems: advances, challenges and prospects.Frontiers in immunology · 2024Review
- Microneedle-mediated treatment for superficial tumors by combining multiple strategies.Drug delivery and translational research · 2023Review
- Microneedle system for tissue engineering and regenerative medicine.Exploration (Beijing, China) · 2023Review
- Recent advances in porous microneedles: materials, fabrication, and transdermal applications.Drug delivery and translational research · 2022Review
- Microneedle systems for delivering nucleic acid drugs.Journal of pharmaceutical investigation · 2022Review
- Laser-Induced Forward Transfer Printing on Microneedles for Transdermal Delivery of Gemcitabine.International journal of bioprinting · 2022Article
- 3D Printing-A "Touch-Button" Approach to Manufacture Microneedles for Transdermal Drug Delivery.Pharmaceutics · 2021Review
- Engineering Microneedle Patches for Improved Penetration: Analysis, Skin Models and Factors Affecting Needle Insertion.Nano-micro letters · 2021Review
- Optimisation of Design and Manufacturing Parameters of 3D Printed Solid Microneedles for Improved Strength, Sharpness, and Drug Delivery.Micromachines · 2021Article
- An overview of microneedle applications, materials, and fabrication methods.Beilstein journal of nanotechnology · 2021Review
- Microneedles: Characteristics, Materials, Production Methods and Commercial Development.Micromachines · 2020Review
- Microneedle Coating Methods: A Review with a Perspective.The Journal of pharmacology and experimental therapeutics · 2019Review
- Immediate separation of microneedle tips from base array during skin insertion for instantaneous drug delivery.RSC advances · 2018Article
- Recent advances of controlled drug delivery using microfluidic platforms.Advanced drug delivery reviews · 2018Review
- Allometric scaling of skin thickness, elasticity, viscoelasticity to mass for micro-medical device translation: from mice, rats, rabbits, pigs to humans.Scientific reports · 2017Article
- Dissolving microneedles for DNA vaccination: Improving functionality via polymer characterization and RALA complexation.Human vaccines & immunotherapeutics · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Microneedle delivery of nucleic acids, in particular plasmid DNA (pDNA), to the skin represents a potential new approach for the clinical management of genetic skin diseases and cutaneous cancers, and for intracutaneous genetic immunisation. In this study excised human skin explants were used to investigate and optimise key parameters that will determine stable and effective microneedle-facilitated pDNA delivery. These include (i) high dose-loading of pDNA onto microneedle surfaces, (ii) stability and functionality of the coated pDNA, (iii) skin penetration capability of pDNA-coated microneedles, and (iv) efficient gene expression in human skin. Optimisation of a dip-coating method enabled significant increases in the loading capacity, up to 100μg of pDNA per 5-microneedle array. Coated microneedles were able to reproducibly perforate human skin at low (<1N) insertion forces. The physical stability of the coated pDNA was partially compromised on storage, although this was improved through the addition of saccharide excipients without detriment to the biological functionality of pDNA. The pDNA-coated microneedles facilitated reporter gene expression in viable human skin. The efficiency of gene expression from coated microneedles will depend upon suitable DNA loading, efficient and reproducible skin puncture and rapid in situ dissolution of the plasmid at the site of delivery.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.