ArticleThe journal of physical chemistry. A2012
Mechanistic insight into the structure and dynamics of entangled and hydrated λ-phage DNA.
Article in The journal of physical chemistry. A, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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3 citing papers in PubMed, 7 citations in OpenAlex.
- Altitudinal Heterogeneity of UV Adaptation inJournal of fungi (Basel, Switzerland) · 2021Article
- Ionic switch controls the DNA state in phage λ.Nucleic acids research · 2015Article
- Solid-to-fluid-like DNA transition in viruses facilitates infection.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
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9 authors at 2 institutions in 2 countries.
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Abstract
Intrinsic dynamics of DNA plays a crucial role in DNA-protein interactions and has been emphasized as a possible key component for in vivo chromatin organization. We have prepared an entangled DNA microtube above the overlap concentration by exploiting the complementary cohesive ends of λ-phage DNA, which is confirmed by atomic force microscopy and agarose gel electrophoresis. Photon correlation spectroscopy further confirmed that the entangled solutions are found to exhibit the classical hydrodynamics of a single chain segment on length scales smaller than the hydrodynamic length scale of single λ-phage DNA molecule. We also observed that in 41.6% (gm water/gm DNA) hydrated state, λ-phage DNA exhibits a dynamic transition temperature (T(dt)) at 187 K and a crossover temperature (T(c)) at 246 K. Computational insight reveals that the observed structure and dynamics of entangled λ-phage DNA are distinctively different from the behavior of the corresponding unentangled DNA with open cohesive ends, which is reminiscent with our experimental observation.
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