ArticlePloS one2012
Alteration of proteins and pigments influence the function of photosystem I under iron deficiency from Chlamydomonas reinhardtii.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 51 citations in OpenAlex.
- Photosystem I structural fusion with IsiA enhances light-harvesting capabilities of filamentous cyanobacterium during iron deficiency.Cell reports. Physical science · 2026Article
- Functional Analysis ofCurrent issues in molecular biology · 2025Article
- A distinct LHCI arrangement is recruited to photosystem I in Fe-starved green algae.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Moderate levels of dissolved iron stimulate cellular growth and increase lipid storage in Symbiodinium sp.Journal of phycology · 2025Article
- Light-dark dependent changes in chloroplast and mitochondrial activity inFrontiers in plant science · 2025Article
- Fe starvation induces a second LHCI tetramer to photosystem I in green algae.bioRxiv : the preprint server for biology · 2024Article
- Chlamydomonas cells transition through distinct Fe nutrition stages within 48 h of transfer to Fe-free medium.Photosynthesis research · 2024Article
- Transcriptome analysis of the bloom-forming dinoflagellate Prorocentrum donghaiense exposed to Ginkgo biloba leaf extract, with an emphasis on photosynthesis.Environmental science and pollution research international · 2024Article
- Long- and short-term acclimation of the photosynthetic apparatus to salinity inFrontiers in plant science · 2023Article
- Research progress on iron absorption, transport, and molecular regulation strategy in plants.Frontiers in plant science · 2023Review
- Silicon mitigates iron deficiency in two energy cane cultivars by modulating physiological and nutritional mechanisms.Frontiers in plant science · 2023Article
- Article
- Enhanced Lipid Production inFrontiers in plant science · 2021Article
- Effects of iron deficiency and exogenous sucrose on the intermediates of chlorophyll biosynthesis in Malus halliana.PloS one · 2020Article
- The Antarctic psychrophiles Chlamydomonas spp. UWO241 and ICE-MDV exhibit differential restructuring of photosystem I in response to iron.Photosynthesis research · 2019Article
- Non-photochemical quenching-dependent acclimation and thylakoid organization of Chlamydomonas reinhardtii to high light stress.Photosynthesis research · 2019Article
- Changes in the photosynthetic apparatus and lipid droplet formation in Chlamydomonas reinhardtii under iron deficiency.Photosynthesis research · 2019Article
- Transcriptional and physiological analyses of short-term Iron deficiency response in apple seedlings provide insight into the regulation involved in photosynthesis.BMC genomics · 2018Article
- Silicon deposition in roots minimizes the cadmium accumulation and oxidative stress in leaves of cowpea plants.Physiology and molecular biology of plants : an international journal of functional plant biology · 2018Article
- Dark Septate Endophytic Fungi Increase Green Manure-Frontiers in plant science · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIron is an essential micronutrient for all organisms because it is a component of enzyme cofactors that catalyze redox reactions in fundamental metabolic processes. Even though iron is abundant on earth, it is often present in the insoluble ferric [Fe (III)] state, leaving many surface environments Fe-limited. The haploid green alga Chlamydomonas reinhardtii is used as a model organism for studying eukaryotic photosynthesis. This study explores structural and functional changes in PSI-LHCI supercomplexes under Fe deficiency as the eukaryotic photosynthetic apparatus adapts to Fe deficiency.
results77K emission spectra and sucrose density gradient data show that PSI and LHCI subunits are affected under iron deficiency conditions. The visible circular dichroism (CD) spectra associated with strongly-coupled chlorophyll dimers increases in intensity. The change in CD signals of pigments originates from the modification of interactions between pigment molecules. Evidence from sucrose gradients and non-denaturing (green) gels indicates that PSI-LHCI levels were reduced after cells were grown for 72 h in Fe-deficient medium. Ultrafast fluorescence spectroscopy suggests that red-shifted pigments in the PSI-LHCI antenna were lost during Fe stress. Further, denaturing gel electrophoresis and immunoblot analysis reveals that levels of the PSI subunits PsaC and PsaD decreased, while PsaE was completely absent after Fe stress. The light harvesting complexes were also susceptible to iron deficiency, with Lhca1 and Lhca9 showing the most dramatic decreases. These changes in the number and composition of PSI-LHCI supercomplexes may be caused by reactive oxygen species, which increase under Fe deficiency conditions.
conclusionsFe deficiency induces rapid reduction of the levels of photosynthetic pigments due to a decrease in chlorophyll synthesis. Chlorophyll is important not only as a light-harvesting pigment, but also has a structural role, particularly in the pigment-rich LHCI subunits. The reduced level of chlorophyll molecules inhibits the formation of large PSI-LHCI supercomplexes, further decreasing the photosynthetic efficiency.
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