ArticleMicroscopy research and technique2012
Measurement of distance with the nanoscale precise imaging by rapid beam oscillation method.
Article in Microscopy research and technique, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
7 citing papers in PubMed, 10 citations in OpenAlex.
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- Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures.Nature communications · 2018Article
- 3D motion of vesicles along microtubules helps them to circumvent obstacles in cells.Journal of cell science · 2017Article
- Phasor Analysis of Local ICS Detects Heterogeneity in Size and Number of Intracellular Vesicles.Biophysical journal · 2016Article
- Orbital Single Particle Tracking on a commercial confocal microscope using piezoelectric stage feedback.Methods and applications in fluorescence · 2014Article
- Axial super resolution topography of focal adhesion by confocal microscopy.Microscopy research and technique · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
We discuss here the principles of a novel optical method in which the scanning of a laser spot around a fluorescent object is used to determine its shape, orientation, and fluorophore distribution. The scanning pattern is adapted to the shape of the object according to a feedback principle based on intensity modulation measurements. The modulation of the intensity with respect to the angular coordinate is used to keep the orbit centered on the object. The modulation induced by rapid oscillations of the orbit radius is used to measure the local distance from the surface with nanometer precision. We provide a model to describe the fundamental relationship between modulation and distance and discuss the range of validity of several approximate expressions. According to this model, the distance can be measured with a precision dependent on the steepness of the point spread function and the total number of detected photons. To test our findings, we performed experiments with one or two channels on fluorescent spheres of known size and characterized the modulation function of our microscope setup. We conclude that the method can be used to measure distances in the range 10-200 nm between two surfaces labeled with two different probes.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.