ArticleDevelopment (Cambridge, England)2012
Sim2 prevents entry into the myogenic program by repressing MyoD transcription during limb embryonic myogenesis.
Article in Development (Cambridge, England), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- The myoblast methylome: multiple types of associations with chromatin and transcription.Epigenetics · 2025Article
- The limb dorsoventral axis: Lmx1b's role in development, pathology, evolution, and regeneration.Developmental dynamics : an official publication of the American Association of Anatomists · 2024Review
- Eutherian-Specific Functions of BetaM Acquired throughLife (Basel, Switzerland) · 2023Article
- Unexpected contribution of fibroblasts to muscle lineage as a mechanism for limb muscle patterning.Nature communications · 2021Article
- DNA methylation changes in Down syndrome derived neural iPSCs uncover co-dysregulation of ZNF and HOX3 families of transcription factors.Clinical epigenetics · 2020Article
- Master control: transcriptional regulation of mammalian Myod.Journal of muscle research and cell motility · 2019Review
- Taiji: System-level identification of key transcription factors reveals transcriptional waves in mouse embryonic development.Science advances · 2019Article
- Article
- Making muscle: Morphogenetic movements and molecular mechanisms of myogenesis in Xenopus laevis.Seminars in cell & developmental biology · 2016Review
- Fine-tuning the onset of myogenesis by homeobox proteins that interact with the Myf5 limb enhancer.Biology open · 2015Article
- Stable and bicistronic expression of two genes in somite- and lateral plate-derived tissues to study chick limb development.BMC developmental biology · 2015Article
- Heparanase 2, mutated in urofacial syndrome, mediates peripheral neural development in Xenopus.Human molecular genetics · 2014Article
- The roles of HLH transcription factors in epithelial mesenchymal transition and multiple molecular mechanisms.Clinical & experimental metastasis · 2014Review
- bHLH-PAS proteins in cancer.Nature reviews. Cancer · 2013Review
- Transcription factor EGR1 directs tendon differentiation and promotes tendon repair.The Journal of clinical investigation · 2013Article
- Article
- Article
- Sonic hedgehog acts cell-autonomously on muscle precursor cells to generate limb muscle diversity.Genes & development · 2012Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
The basic helix-loop-helix transcription factor MyoD is a central actor that triggers the skeletal myogenic program. Cell-autonomous and non-cell-autonomous regulatory pathways must tightly control MyoD expression to ensure correct initiation of the muscle program at different places in the embryo and at different developmental times. In the present study, we have addressed the involvement of Sim2 (single-minded 2) in limb embryonic myogenesis. Sim2 is a bHLH-PAS transcription factor that inhibits transcription by active repression and displays enhanced expression in ventral limb muscle masses during chick and mouse embryonic myogenesis. We have demonstrated that Sim2 is expressed in muscle progenitors that have not entered the myogenic program, in different experimental conditions. MyoD expression is transiently upregulated in limb muscle masses of Sim2(-/-) mice. Conversely, Sim2 gain-of-function experiments in chick and Xenopus embryos showed that Sim2 represses MyoD expression. In addition, we show that Sim2 represses the activity of the mouse MyoD promoter in primary myoblasts and is recruited to the MyoD core enhancer in embryonic mouse limbs. Sim2 expression is non-autonomously and negatively regulated by the dorsalising factor Lmx1b. We propose that Sim2 represses MyoD transcription in limb muscle masses, through Sim2 recruitment to the MyoD core enhancer, in order to prevent premature entry into the myogenic program. This MyoD repression is predominant in ventral limb regions and is likely to contribute to the differential increase of the global mass of ventral muscles versus dorsal muscles.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.