Evidence map›Paper›PMID 22513293›Full record

ArticlePancreas2012

Plasma Shh levels reduced in pancreatic cancer patients.

Mohamad El-Zaatari, Stephanie Daignault, Art Tessier, Gail Kelsey, Lisa A Travnikar, Esperanza F Cantu, Jamie Lee, Caitlyn M Plonka, Diane M Simeone, Michelle A Anderson and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pancreas, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.7field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 15 citations in OpenAlex.

  1. Randomized Phase Ib/II Study of Gemcitabine Plus Placebo or Vismodegib, a Hedgehog Pathway Inhibitor, in Patients With Metastatic Pancreatic Cancer.Journal of clinical oncology : official journal of the American Society of Clinical Oncology · 2015
    Trial
  2. Systemic Sonic Hedgehog Signaling Links Intestinal Nutrient Sensing With Sex-Specific Type 2 Diabetes Progression.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026
    Article
  3. Article
  4. Hedgehog Signaling Links Chronic Inflammation to Gastric Cancer Precursor Lesions.Cellular and molecular gastroenterology and hepatology · 2017
    Review
  5. Review
  6. Review
  7. Review
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Mohamad El-ZaatariDepartment of Internal Medicine-Gastroenterology, University of Michigan, Ann Arbor, MI 48109-2200, USA
Stephanie Daignault
Art Tessier
Gail Kelsey
Lisa A Travnikar
Esperanza F Cantu
Jamie Lee
Caitlyn M Plonka
Diane M Simeone
Michelle A Anderson
Juanita L Merchant
University of Michigan–Ann Arbor · US

Funding

XenograftP30CA046592 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI THEODORE S LAWRENCE · 1988 to 2026
$178.2M
MICHIGAN INSTITUTE FOR CLINICAL AND HEALTH RESEARCH (MICHR) (UL1): (BPCA)UL1RR024986 · NCRR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI SHANLEY, THOMAS P. · 2007 to 2011
$51.2M
Role of Hedgehog Signaling in Chronic Gastritis and MetaplasiaP01DK062041 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI MERCHANT, JUANITA L. · 2002 to 2017
$20.6M
Validation Study of Select Biomarkers for the Diagnostic of Pancreatic CancerK23DK082097 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI ANDERSON, MICHELLE ANN · 2008 to 2012
$902k
NCI NIH HHS P30 CA046592NCI NIH HHS P30 CA46592NCRR NIH HHS UL1 RR024986NCRR NIH HHS UL1RR024986NIDDK NIH HHS K23 DK082097NIDDK NIH HHS K23-DK082097NIDDK NIH HHS P01 DK062041NIDDK NIH HHS P01-DK62041
6 · The paper itself

Abstract

objectivesNormally, sonic hedgehog (Shh) is expressed in the pancreas during fetal development and transiently after tissue injury. Although pancreatic cancers express Shh, it is not known if the protein is secreted into the blood and whether its plasma levels change with pancreatic transformation. The goal of this study was to develop an enzyme-linked immunosorbent assay to detect human Shh in blood and determine its levels in subjects with and without pancreatic cancer.

methodsA human Shh enzyme-linked immunosorbent assay was developed, and plasma Shh levels were measured in blood samples from healthy subjects and patients with pancreatitis or pancreatic cancer. The biological activity of plasma Shh was tested using NIH-3T3 cells.

resultsThe mean levels of Shh in human blood were lower in patients with pancreatitis and pancreatic cancer than in healthy subjects. Hematopoietic cells did not express Shh, suggesting that Shh is secreted into the bloodstream. Plasma fractions enriched with Shh did not induce Gli-1 messenger RNA, suggesting that the protein was not biologically active.

conclusionsShh is secreted from tissues and organs into the circulation, but its activity is blocked by plasma proteins. Reduced plasma levels were found in pancreatic cancer patients, but alone were not sufficient to predict pancreatic cancer.

Indexed as

AdolescentAdultAgedAnimalsBlood ProteinsEnzyme-Linked Immunosorbent AssayFemaleFibrosisGene ExpressionHedgehog ProteinsHumansKruppel-Like Transcription FactorsMaleMiceMiddle AgedNIH 3T3 CellsBlood ProteinsGli1 protein, mouseHedgehog ProteinsKruppel-Like Transcription FactorsSHH protein, humanZinc Finger Protein GLI1

Identifiers

PMID22513293
PMCPMC3404255
OpenAlexW2316089511

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.