ArticleBiological procedures online2012
Competitive DNA transfection formulation via electroporation for human adipose stem cells and mesenchymal stem cells.
Article in Biological procedures online, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Who cites it
9 citing papers in PubMed.
- Non-viral gene delivery to human mesenchymal stem cells: a practical guide towards cell engineering.Journal of biological engineering · 2023Review
- Electroporation: A Sustainable and Cell Biology Preserving Cell Labeling Method for Adipogenous Mesenchymal Stem Cells.BioResearch open access · 2019Article
- Single-step electrical field strength screening to determine electroporation induced transmembrane transport parameters.Biochimica et biophysica acta · 2016Article
- Efficient, Low-Cost Nucleofection of Passaged Chondrocytes.Cartilage · 2016Article
- Comparison of Different Electroporation Parameters on Transfection Efficiency of Sheep Testicular Cells.Cell journal · 2016Article
- "Strategic sequences" in adipose-derived stem cell nerve regeneration.Microsurgery · 2014Review
- The impact of non-electrical factors on electrical gene transfer.Methods in molecular biology (Clifton, N.J.) · 2014Review
- Physical non-viral gene delivery methods for tissue engineering.Annals of biomedical engineering · 2013Review
- Direct head-to-head comparison of cationic liposome-mediated gene delivery to mesenchymal stem/stromal cells of different human sources: a comprehensive study.Human gene therapy methods · 2013Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
backgroundAdipose stem cells have a strong potential for use in cell-based therapy, but the current nucleofection technique, which relies on unknown buffers, prevents their use.
resultsWe developed an optimal nucleofection formulation for human adipose stem cells by using a three-step method that we had developed previously. This method was designed to determine the optimal formulation for nucleofection that was capable of meeting or surpassing the established commercial buffer (Amaxa), in particular for murine adipose stem cells. By using this same buffer, we determined that the same formulation yields optimal transfection efficiency in human mesenchymal stem cells.
conclusionsOur findings suggest that transfection efficiency in human stem cells can be boosted with proper formulation.
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Registered trials
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