ArticlePloS one2012
Mechanisms underlying the confined diffusion of cholera toxin B-subunit in intact cell membranes.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 63 citations in OpenAlex.
- A paintbrush for delivery of nanoparticles and molecules to live cells with precise spatiotemporal control.Nature methods · 2024Article
- Lipid Peroxidation Drives Liquid-Liquid Phase Separation and Disrupts Raft Protein Partitioning in Biological Membranes.Journal of the American Chemical Society · 2024Article
- Antibody binding reports spatial heterogeneities in cell membrane organization.Nature communications · 2023Article
- The Utility of Fluorescence Recovery after Photobleaching (FRAP) to Study the Plasma Membrane.Membranes · 2023Review
- Structural basis for acyl chain control over glycosphingolipid sorting and vesicular trafficking.Cell reports · 2022Article
- Regression Analysis of Confocal FRAP and its Application to Diffusion in Membranes.Journal of fluorescence · 2022Article
- Three-dimensional localization refinement and motion model parameter estimation for confined single particle tracking under low-light conditions.Biomedical optics express · 2021Article
- Pore Forming Protein Induced Biomembrane Reorganization and Dynamics: A Focused Review.Frontiers in molecular biosciences · 2021Review
- Structured clustering of the glycosphingolipid GM1 is required for membrane curvature induced by cholera toxin.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Disruption of palmitate-mediated localization; a shared pathway of force and anesthetic activation of TREK-1 channels.Biochimica et biophysica acta. Biomembranes · 2020Review
- SPT and Imaging FCS Provide Complementary Information on the Dynamics of Plasma Membrane Molecules.Biophysical journal · 2018Article
- Fucosylated Molecules Competitively Interfere with Cholera Toxin Binding to Host Cells.ACS infectious diseases · 2018Article
- Hetero-multivalent binding of cholera toxin subunit B with glycolipid mixtures.Colloids and surfaces. B, Biointerfaces · 2017Article
- Nanoscale Membrane Budding Induced by CTxB and Detected via Polarized Localization Microscopy.Biophysical journal · 2017Article
- Supramolecular Nanofibers Enhance Growth Factor Signaling by Increasing Lipid Raft Mobility.Nano letters · 2016Article
- Confined diffusion of transmembrane proteins and lipids induced by the same actin meshwork lining the plasma membrane.Molecular biology of the cell · 2016Article
- Cross-linking of glycosphingolipids at the plasma membrane: consequences for intracellular signaling and traffic.Cellular and molecular life sciences : CMLS · 2016Article
- Sphingolipid Organization in the Plasma Membrane and the Mechanisms That Influence It.Frontiers in cell and developmental biology · 2016Review
- n-3 polyunsaturated fatty acids suppress CD4(+) T cell proliferation by altering phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2] organization.Biochimica et biophysica acta · 2016Article
- Diffusion of GPI-anchored proteins is influenced by the activity of dynamic cortical actin.Molecular biology of the cell · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Multivalent glycolipid binding toxins such as cholera toxin have the capacity to cluster glycolipids, a process thought to be important for their functional uptake into cells. In contrast to the highly dynamic properties of lipid probes and many lipid-anchored proteins, the B-subunit of cholera toxin (CTxB) diffuses extremely slowly when bound to its glycolipid receptor GM(1) in the plasma membrane of living cells. In the current study, we used confocal FRAP to examine the origins of this slow diffusion of the CTxB/GM(1) complex at the cell surface, relative to the behavior of a representative GPI-anchored protein, transmembrane protein, and fluorescent lipid analog. We show that the diffusion of CTxB is impeded by actin- and ATP-dependent processes, but is unaffected by caveolae. At physiological temperature, the diffusion of several cell surface markers is unchanged in the presence of CTxB, suggesting that binding of CTxB to membranes does not alter the organization of the plasma membrane in a way that influences the diffusion of other molecules. Furthermore, diffusion of the B-subunit of another glycolipid-binding toxin, Shiga toxin, is significantly faster than that of CTxB, indicating that the confined diffusion of CTxB is not a simple function of its ability to cluster glycolipids. By identifying underlying mechanisms that control CTxB dynamics at the cell surface, these findings help to delineate the fundamental properties of toxin-receptor complexes in intact cell membranes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.