ArticlePLoS genetics2012
Context-dependent dual role of SKI8 homologs in mRNA synthesis and turnover.
Article in PLoS genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- The plant early recombinosome: a high security complex to break DNA during meiosis.Plant reproduction · 2024Review
- Regulation of maize growth and immunity by ZmSKI3-mediated RNA decay and post-transcriptional gene silencing.Journal of integrative plant biology · 2024Article
- Article
- The plant POLYMERASE-ASSOCIATED FACTOR1 complex links transcription and H2B monoubiquitination genome wide.Plant physiology · 2024Article
- Increased gene expression variability hinders the formation of regional mechanical conflicts leading to reduced organ shape robustness.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Day-night and seasonal variation of human gene expression across tissues.PLoS biology · 2023Article
- Nuclear and cytoplasmic RNA exosomes and PELOTA1 prevent miRNA-induced secondary siRNA production in Arabidopsis.Nucleic acids research · 2022Article
- Day-night and seasonal variation of human gene expression across tissues.bioRxiv : the preprint server for biology · 2022Article
- RNA Exosomes and Their Cofactors.Methods in molecular biology (Clifton, N.J.) · 2020Review
- Paf1c defects challenge the robustness of flower meristem termination inDevelopment (Cambridge, England) · 2019Article
- RST1 and RIPR connect the cytosolic RNA exosome to the Ski complex in Arabidopsis.Nature communications · 2019Article
- Loss of function of a rice TPR-domain RNA-binding protein confers broad-spectrum disease resistance.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Polysomes, Stress Granules, and Processing Bodies: A Dynamic Triumvirate Controlling Cytoplasmic mRNA Fate and Function.Plant physiology · 2018Review
- Phyllotactic regularity requires the Paf1 complex inDevelopment (Cambridge, England) · 2017Article
- The RNA Polymerase-Associated Factor 1 Complex Is Required for Plant Touch Responses.Journal of experimental botany · 2017Article
- SUPERKILLER Complex Components Are Required for the RNA Exosome-Mediated Control of Cuticular Wax Biosynthesis in Arabidopsis Inflorescence Stems.Plant physiology · 2016Article
- SKI2 mediates degradation of RISC 5'-cleavage fragments and prevents secondary siRNA production from miRNA targets in Arabidopsis.Nucleic acids research · 2015Article
- The RNA helicases AtMTR4 and HEN2 target specific subsets of nuclear transcripts for degradation by the nuclear exosome in Arabidopsis thaliana.PLoS genetics · 2014Article
- Threading the barrel of the RNA exosome.Trends in biochemical sciences · 2013Review
- Suppression of Arabidopsis protophloem differentiation and root meristem growth by CLE45 requires the receptor-like kinase BAM3.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eukaryotic mRNA transcription and turnover is controlled by an enzymatic machinery that includes RNA polymerase II and the 3' to 5' exosome. The activity of these protein complexes is modulated by additional factors, such as the nuclear RNA polymerase II-associated factor 1 (Paf1c) and the cytoplasmic Superkiller (SKI) complex, respectively. Their components are conserved across uni- as well as multi-cellular organisms, including yeast, Arabidopsis, and humans. Among them, SKI8 displays multiple facets on top of its cytoplasmic role in the SKI complex. For instance, nuclear yeast ScSKI8 has an additional function in meiotic recombination, whereas nuclear human hSKI8 (unlike ScSKI8) associates with Paf1c. The Arabidopsis SKI8 homolog VERNALIZATION INDEPENDENT 3 (VIP3) has been found in Paf1c as well; however, whether it also has a role in the SKI complex remains obscure so far. We found that transgenic VIP3-GFP, which complements a novel vip3 mutant allele, localizes to both nucleus and cytoplasm. Consistently, biochemical analyses suggest that VIP3-GFP associates with the SKI complex. A role of VIP3 in the turnover of nuclear encoded mRNAs is supported by random-primed RNA sequencing of wild-type and vip3 seedlings, which indicates mRNA stabilization in vip3. Another SKI subunit homolog mutant, ski2, displays a dwarf phenotype similar to vip3. However, unlike vip3, it displays neither early flowering nor flower development phenotypes, suggesting that the latter reflect VIP3's role in Paf1c. Surprisingly then, transgenic ScSKI8 rescued all aspects of the vip3 phenotype, suggesting that the dual role of SKI8 depends on species-specific cellular context.
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Registered trials
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