ArticleThe Journal of biological chemistry2012
Dimerization of plant defensin NaD1 enhances its antifungal activity.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 76 citations in OpenAlex.
- Antimicrobial peptides in the era of resistance: integrating AI-driven design, host-microbe interactions, and nanotechnological delivery.Frontiers in microbiology · 2026Review
- Plant Defensin PgD1 a Biotechnological Alternative Against Plant Pathogens.Probiotics and antimicrobial proteins · 2025Article
- The defensin gene NaDLP1, which confers resistance against Alternaria alternata, is transcriptionally regulated by NaWRKY3/6 in Nicotiana attenuata.BMC plant biology · 2025Article
- Cylindracin, a Fruiting Body-Specific Protein ofToxins · 2025Article
- Hyperpolarisation of Mitochondrial Membranes Is a Critical Component of the Antifungal Mechanism of the Plant Defensin, Ppdef1.Journal of fungi (Basel, Switzerland) · 2024Article
- The Plant Defensin Ppdef1 Is a Novel Topical Treatment for Onychomycosis.Journal of fungi (Basel, Switzerland) · 2023Article
- Engineering defensin α-helix to produce high-affinity SARS-CoV-2 spike protein binding ligands.Protein science : a publication of the Protein Society · 2022Article
- Defensin-lipid interactions in membrane targeting: mechanisms of action and opportunities for the development of antimicrobial and anticancer therapeutics.Biochemical Society transactions · 2022Review
- Use of Defensins to Develop Eco-Friendly Alternatives to Synthetic Fungicides to Control Phytopathogenic Fungi and Their Mycotoxins.Journal of fungi (Basel, Switzerland) · 2022Review
- Tick defensin γ-core reduces Fusarium graminearum growth and abrogates mycotoxins production with high efficiency.Scientific reports · 2021Article
- Article
- Membrane-Interacting Antifungal Peptides.Frontiers in cell and developmental biology · 2021Review
- Structural Diversity and Highly Specific Host-Pathogen Transcriptional Regulation of Defensin Genes Is Revealed in Tomato.International journal of molecular sciences · 2020Article
- Histidine-Rich Defensins from theJournal of fungi (Basel, Switzerland) · 2020Article
- Plant Defensins from a Structural Perspective.International journal of molecular sciences · 2020Review
- The interaction with fungal cell wall polysaccharides determines the salt tolerance of antifungal plant defensins.Cell surface (Amsterdam, Netherlands) · 2019Article
- Article
- Screening theAntimicrobial agents and chemotherapy · 2019Article
- Plant defensins: types, mechanism of action and prospects of genetic engineering for enhanced disease resistance in plants.3 Biotech · 2019Review
- Salt-Tolerant Antifungal and Antibacterial Activities of the Corn Defensin ZmD32.Frontiers in microbiology · 2019Article
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plant defensin, NaD1, from the flowers of Nicotiana alata, is a member of a family of cationic peptides that displays growth inhibitory activity against several filamentous fungi, including Fusarium oxysporum. The antifungal activity of NaD1 has been attributed to its ability to permeabilize membranes; however, the molecular basis of this function remains poorly defined. In this study, we have solved the structure of NaD1 from two crystal forms to high resolution (1.4 and 1.58 Å, respectively), both of which contain NaD1 in a dimeric configuration. Using protein cross-linking experiments as well as small angle x-ray scattering analysis and analytical ultracentrifugation, we show that NaD1 forms dimers in solution. The structural studies identified Lys(4) as critical in formation of the NaD1 dimer. This was confirmed by site-directed mutagenesis of Lys(4) that resulted in substantially reduced dimer formation. Significantly, the reduced ability of the Lys(4) mutant to dimerize correlated with diminished antifungal activity. These data demonstrate the importance of dimerization in NaD1 function and have implications for the use of defensins in agribiotechnology applications such as enhancing plant crop protection against fungal pathogens.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.