ArticleThe Journal of biological chemistry2012
Signal-dependent slow leukocyte rolling does not require cytoskeletal anchorage of P-selectin glycoprotein ligand-1 (PSGL-1) or integrin αLβ2.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 33 citations in OpenAlex.
- Platelet-derived integrin- and tetraspanin-enriched tethers exacerbate severe inflammation.Science (New York, N.Y.) · 2026Article
- Deletion of Talin1 in Myeloid Cells Facilitates Atherosclerosis in Mice.Arteriosclerosis, thrombosis, and vascular biology · 2024Article
- Review
- Integrin Regulators in Neutrophils.Cells · 2022Review
- Mitofusin-2 regulates leukocyte adhesion and β2 integrin activation.Journal of leukocyte biology · 2022Article
- Biphasic Force-Regulated Phosphorylation Site Exposure and Unligation of ERM Bound with PSGL-1: A Novel Insight into PSGL-1 Signaling via Steered Molecular Dynamics Simulations.International journal of molecular sciences · 2020Article
- Neutrophils lacking ERM proteins polarize and crawl directionally but have decreased adhesion strength.Blood advances · 2020Article
- Th1 Cells Rolling on Selectins Trigger DAP12-Dependent Signals That Activate Integrin αLβ2.Journal of immunology (Baltimore, Md. : 1950) · 2020Article
- Adhesive dynamics simulations quantitatively predict effects of kindlin-3 deficiency on T-cell homing.Integrative biology : quantitative biosciences from nano to macro · 2019Article
- Cooperative PSGL-1 and CXCR2 signaling in neutrophils promotes deep vein thrombosis in mice.Blood · 2018Article
- Thyroid cancer cells in space during the TEXUS-53 sounding rocket mission - The THYROID Project.Scientific reports · 2018Article
- Microfluidics-based super-resolution microscopy enables nanoscopic characterization of blood stem cell rolling.Science advances · 2018Article
- Selectins and chemokines use shared and distinct signals to activate β2 integrins in neutrophils.Blood advances · 2018Article
- An Experimentally Determined State Diagram for Human CD4Cellular and molecular bioengineering · 2018Article
- Multi-Inhibitory Effects of A2A Adenosine Receptor Signaling on Neutrophil Adhesion Under Flow.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Selectins: initiators of leucocyte adhesion and signalling at the vascular wall.Cardiovascular research · 2015Review
- Blocking neutrophil integrin activation prevents ischemia-reperfusion injury.The Journal of experimental medicine · 2015Article
- O-glycans direct selectin ligands to lipid rafts on leukocytes.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Cytoskeletal regulation of CD44 membrane organization and interactions with E-selectin.The Journal of biological chemistry · 2014Article
- Expression of CD11b (MAC-1) and CD162 (PSGL-1) on monocytes is decreased under conditions of deep hypothermic circulatory arrest.Experimental and therapeutic medicine · 2014Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
In inflamed venules, neutrophils roll on P- or E-selectin, engage P-selectin glycoprotein ligand-1 (PSGL-1), and signal extension of integrin α(L)β(2) in a low affinity state to slow rolling on intercellular adhesion molecule-1 (ICAM-1). Cytoskeleton-dependent receptor clustering often triggers signaling, and it has been hypothesized that the cytoplasmic domain links PSGL-1 to the cytoskeleton. Chemokines cause rolling neutrophils to fully activate α(L)β(2), leading to arrest on ICAM-1. Cytoskeletal anchorage of α(L)β(2) has been linked to chemokine-triggered extension and force-regulated conversion to the high affinity state. We asked whether PSGL-1 must interact with the cytoskeleton to initiate signaling and whether α(L)β(2) must interact with the cytoskeleton to extend. Fluorescence recovery after photobleaching of transfected cells documented cytoskeletal restraint of PSGL-1. The lateral mobility of PSGL-1 similarly increased by depolymerizing actin filaments with latrunculin B or by mutating the cytoplasmic tail to impair binding to the cytoskeleton. Converting dimeric PSGL-1 to a monomer by replacing its transmembrane domain did not alter its mobility. By transducing retroviruses expressing WT or mutant PSGL-1 into bone marrow-derived macrophages from PSGL-1-deficient mice, we show that PSGL-1 required neither dimerization nor cytoskeletal anchorage to signal β(2) integrin-dependent slow rolling on P-selectin and ICAM-1. Depolymerizing actin filaments or decreasing actomyosin tension in neutrophils did not impair PSGL-1- or chemokine-mediated integrin extension. Unlike chemokines, PSGL-1 did not signal cytoskeleton-dependent swing out of the β(2)-hybrid domain associated with the high affinity state. The cytoskeletal independence of PSGL-1-initiated, α(L)β(2)-mediated slow rolling differs markedly from the cytoskeletal dependence of chemokine-initiated, α(L)β(2)-mediated arrest.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.