ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2012
An image analysis method for the precise selection and quantitation of fluorescently labeled cellular constituents: application to the measurement of human muscle cells in culture.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- A Biomimetic 3D Human Skeletal Muscle Microtissue for Modeling Biological and Functional Hallmarks of Aging.Small science · 2026Article
- Hypoxia enhances human myoblast differentiation: involvement of HIF1α and impact of DUX4, the FSHD causal gene.Skeletal muscle · 2023Article
- Investigating proliferation and differentiation capacities of Hanwoo steer myosatellite cells at different passages for developing cell-cultured meat.Scientific reports · 2023Article
- Silver electroceutical technology to treat sarcopenia.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- VAX014, an Oncolytic Therapy, Reduces Adenomas and Modifies Colon Microenvironment in Mouse Model of CRC.International journal of molecular sciences · 2023Article
- The Use of Collagen Methacrylate in Actuating Polyethylene Glycol Diacrylate-Acrylic Acid Scaffolds for Muscle Regeneration.Annals of biomedical engineering · 2023Article
- Generation and Integrated Analysis of Advanced Patient-Derived Orthoxenograft Models (PDOX) for the Rational Assessment of Targeted Therapies in Endometrial Cancer.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Effects of Hypoxia on Proliferation and Differentiation in Belgian Blue and Hanwoo Muscle Satellite Cells for the Development of Cultured Meat.Biomolecules · 2022Article
- The Effect of Ex Vivo Human Serum from Liver Disease Patients on Cellular Protein Synthesis and Growth.Cells · 2022Article
- Quantification of Muscle Stem Cell Differentiation Using Live-Cell Imaging and Eccentricity Measures.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Identification of a KLF5-dependent program and drug development for skeletal muscle atrophy.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Article
- Epithelial membrane protein 2 (Emp2) modulates innate immune cell population recruitment at the maternal-fetal interface.Journal of reproductive immunology · 2021Article
- Bioinspired electrospun dECM scaffolds guide cell growth and control the formation of myotubes.Science advances · 2021Article
- RGMa can induce skeletal muscle cell hyperplasia via association with neogenin signalling pathway.In vitro cellular & developmental biology. Animal · 2021Article
- New insights on repeated acoustic injury: Augmentation of cochlear susceptibility and inflammatory reaction resultant of prior acoustic injury.Hearing research · 2020Article
- Article
- Graphene oxide nanofilm and the addition of L-glutamine can promote development of embryonic muscle cells.Journal of nanobiotechnology · 2020Article
- MyoCount: a software tool for the automated quantification of myotube surface area and nuclear fusion index.Wellcome open research · 2019Article
- Lower level noise exposure that produces only TTS modulates the immune homeostasis of cochlear macrophages.Journal of neuroimmunology · 2018Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The accurate measurement of the morphological characteristics of cells with nonuniform conformations presents difficulties. We report here a straightforward method using immunofluorescent staining and the commercially available imaging program Adobe Photoshop, which allows objective and precise information to be gathered on irregularly shaped cells. We have applied this measurement technique to the analysis of human muscle cells and their immunologically marked intracellular constituents, as these cells are prone to adopting a highly branched phenotype in culture. Use of this method can be used to overcome many of the long-standing limitations of conventional approaches for quantifying muscle cell size in vitro. In addition, wider applications of Photoshop as a quantitative and semiquantitative tool in immunocytochemistry are explored.
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