ArticleEnvironmental health perspectives2012
Biosensor zebrafish provide new insights into potential health effects of environmental estrogens.
Article in Environmental health perspectives, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 72 citations in OpenAlex.
- Balancing Discovery and Caution: Regeneration in zebrafish toxicology.Current opinion in toxicology · 2026Article
- Zebrafish neuromast development: a target for endocrine disrupting chemicals?Frontiers in toxicology · 2026Article
- Ethanol downregulates gastrula gene expression and cell movement, causing symptoms of foetal alcohol spectrum disorders.Biology open · 2025Article
- Application of transgenic zebrafish for investigating inflammatory responses to nanomaterials: Recommendations for new users.F1000Research · 2023Review
- Zebrafish as an emerging model to study estrogen receptors in neural development.Frontiers in endocrinology · 2023Review
- Estrogens regulate early embryonic development of the olfactory sensory system via estrogen-responsive glia.Development (Cambridge, England) · 2022Article
- Application of Transgenic Zebrafish Models for Studying the Effects of Estrogenic Endocrine Disrupting Chemicals on Embryonic Brain Development.Frontiers in pharmacology · 2022Review
- Health Effects and Life Stage Sensitivities in Zebrafish Exposed to an Estrogenic Wastewater Treatment Works Effluent.Frontiers in endocrinology · 2021Article
- Long Exposure to a Diet Supplemented with Antioxidant and Anti-Inflammatory Probiotics Improves Sperm Quality and Progeny Survival in the Zebrafish Model.Biomolecules · 2019Article
- Cardiovascular Effects and Molecular Mechanisms of Bisphenol A and Its Metabolite MBP in Zebrafish.Environmental science & technology · 2019Article
- Early life exposure to ethinylestradiol enhances subsequent responses to environmental estrogens measured in a novel transgenic zebrafish.Scientific reports · 2018Article
- Review
- Zebrafish: A Model for the Study of Toxicants Affecting Muscle Development and Function.International journal of molecular sciences · 2016Review
- Development of a fluorescent transgenic zebrafish biosensor for sensing aquatic heavy metal pollution.Transgenic research · 2016Article
- Zebrafish biosensor for toxicant induced muscle hyperactivity.Scientific reports · 2016Article
- Zebrafish: A Versatile Animal Model for Fertility Research.BioMed research international · 2016Review
- Assessing variation in the potential susceptibility of fish to pharmaceuticals, considering evolutionary differences in their physiology and ecology.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Article
- Transcriptional analysis of endocrine disruption using zebrafish and massively parallel sequencing.Journal of molecular endocrinology · 2014Review
- Ketamine attenuates cytochrome p450 aromatase gene expression and estradiol-17β levels in zebrafish early life stages.Journal of applied toxicology : JAT · 2014Article
- Transgenic zebrafish reveal tissue-specific differences in estrogen signaling in response to environmental water samples.Environmental health perspectives · 2014Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEnvironmental estrogens alter hormone signaling in the body that can induce reproductive abnormalities in both humans and wildlife. Available testing systems for estrogens are focused on specific systems such as reproduction. Crucially, however, the potential for significant health impacts of environmental estrogen exposures on a variety of body systems may have been overlooked.
objectiveOur aim was to develop and apply a sensitive transgenic zebrafish model to assess real-time effects of environmental estrogens on signaling mechanisms in a whole body system for use in integrated health assessments.
methodsWe created a novel transgenic biosensor zebrafish containing an estrogen-inducible promoter derived with multiple tandem estrogen responsive elements (EREs) and a Gal4ff-UAS system for enhanced response sensitivity.
resultsUsing our novel estrogen-responsive transgenic (TG) zebrafish, we identified target tissues for environmental estrogens; these tissues have very high sensitivity even at environmentally relevant concentrations. Exposure of the TG fish to estrogenic endocrine-disrupting chemicals (EDCs) induced specific expression of green fluorescent protein (GFP) in a wide variety of tissues including the liver, heart, skeletal muscle, otic vesicle, forebrain, lateral line, and ganglions, most of which have not been established previously as targets for estrogens in fish. Furthermore, we found that different EDCs induced GFP expression with different tissue response patterns and time trajectories, suggesting different potential health effects.
conclusionWe have developed a powerful new model for understanding toxicological effects, mechanisms, and health impacts of environmental estrogens in vertebrates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.