ArticleArthritis research & therapy2012
Circulating and synovial antibody profiling of juvenile arthritis patients by nucleic acid programmable protein arrays.
Article in Arthritis research & therapy, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 42 citations in OpenAlex.
- Autoantibody Response Toward Chromatin in Patients With Juvenile Idiopathic Arthritis.Arthritis & rheumatology (Hoboken, N.J.) · 2026Article
- Human antibody profiling technologies for autoimmune disease.Immunologic research · 2023Review
- Identification of novel autoantigens as potential biomarkers in juvenile idiopathic arthritis associated uveitis.Frontiers in pediatrics · 2022Article
- Nucleic Acid Programmable Protein Arrays (NAPPA) for the Discovery of Autoantibodies in Osteoarthritis.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Applications of Protein Microarrays in Biomarker Discovery for Autoimmune Diseases.Frontiers in immunology · 2021Review
- Developments and Applications of Functional Protein Microarrays.Molecular & cellular proteomics : MCP · 2020Review
- Discovery of an autoantibody signature for the early diagnosis of knee osteoarthritis: data from the Osteoarthritis Initiative.Annals of the rheumatic diseases · 2019Article
- Autoantibodies in the Pathogenesis, Diagnosis, and Prognosis of Juvenile Idiopathic Arthritis.Frontiers in immunology · 2018Review
- High-throughput screening of biomolecules using cell-free gene expression systems.Synthetic biology (Oxford, England) · 2018Review
- Identification of disease-specific motifs in the antibody specificity repertoire via next-generation sequencing.Scientific reports · 2016Article
- Autoimmune response to transthyretin in juvenile idiopathic arthritis.JCI insight · 2016Article
- Antiviral antibody profiling by high-density protein arrays.Proteomics · 2015Article
- NAPPA as a Real New Method for Protein Microarray Generation.Microarrays (Basel, Switzerland) · 2015Review
- Host-pathogen interaction profiling using self-assembling human protein arrays.Journal of proteome research · 2015Article
- Microreactor array device.Scientific reports · 2015Article
- New autoantibody detection technologies yield novel insights into autoimmune disease.Current opinion in rheumatology · 2014Review
- Exploration of panviral proteome: high-throughput cloning and functional implications in virus-host interactions.Theranostics · 2014Article
- Autoantibody profiling in multiple sclerosis using arrays of human protein fragments.Molecular & cellular proteomics : MCP · 2013Article
- High density diffusion-free nanowell arrays.Journal of proteome research · 2012Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
introductionJuvenile idiopathic arthritis (JIA) is a heterogeneous disease characterized by chronic joint inflammation of unknown cause in children. JIA is an autoimmune disease and small numbers of autoantibodies have been reported in JIA patients. The identification of antibody markers could improve the existing clinical management of patients.
methodsA pilot study was performed on the application of a high-throughput platform, the nucleic acid programmable protein array (NAPPA), to assess the levels of antibodies present in the systemic circulation and synovial joint of a small cohort of juvenile arthritis patients. Plasma and synovial fluid from 10 JIA patients was screened for antibodies against 768 proteins on NAPPAs.
resultsQuantitative reproducibility of NAPPAs was demonstrated with > 0.95 intra-array and inter-array correlations. A strong correlation was also observed for the levels of antibodies between plasma and synovial fluid across the study cohort (r = 0.96). Differences in the levels of 18 antibodies were revealed between sample types across all patients. Patients were segregated into two clinical subtypes with distinct antibody signatures by unsupervised hierarchical cluster analysis.
conclusionThe NAPPAs provide a high-throughput quantitatively reproducible platform to screen for disease-specific autoantibodies at the proteome level on a microscope slide. The strong correlation between the circulating antibody levels and those of the inflamed joint represents a novel finding and provides confidence to use plasma for discovery of autoantibodies in JIA, thus circumventing the challenges associated with joint aspiration. We expect that autoantibody profiling of JIA patients on NAPPAs could yield antibody markers that can act as criteria to stratify patients, predict outcomes and understand disease etiology at the molecular level.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.