ArticlePloS one2012
The GPI anchor signal sequence dictates the folding and functionality of the Als5 adhesin from Candida albicans.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 17 citations in OpenAlex.
- In Silico Analysis and Expression Profiling of Adhesin-Like Flocculins in Trichosporon asahii.Mycopathologia · 2026Article
- Unveiling the structure of GPI-anchored protein of Malassezia globosa and its pathogenic role in pityriasis versicolor.Journal of molecular modeling · 2021Article
- Ras signaling activates glycosylphosphatidylinositol (GPI) anchor biosynthesis via the GPI-The Journal of biological chemistry · 2018Article
- Fungal Biofilms: Inside Out.Microbiology spectrum · 2017Review
- Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundProteins destined to be Glycosylphosphatidylinositol (GPI) anchored are translocated into the ER lumen completely before the C-terminal GPI anchor attachment signal sequence (SS) is removed by the GPI-transamidase and replaced by a pre-formed GPI anchor precursor. Does the SS have a role in dictating the conformation and function of the protein as well? METHODOLOGY/PRINCIPAL
findingsWe generated two variants of the Als5 protein without and with the SS in order to address the above question. Using a combination of biochemical and biophysical techniques, we show that in the case of Als5, an adhesin of C. albicans, the C-terminal deletion of 20 amino acids (SS) results in a significant alteration in conformation and function of the mature protein. CONCLUSIONS/SIGNIFICANCE: We propose that the locking of the conformation of the precursor protein in an alternate conformation from that of the mature protein is one probable strategy employed by the cell to control the behaviour and function of proteins intended to be GPI anchored during their transit through the ER.
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Registered trials
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