ArticlePloS one2012
An epithelial serine protease, AgESP, is required for Plasmodium invasion in the mosquito Anopheles gambiae.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Genetic control of malaria transmission: a salivary gland-centric perspective.Current opinion in insect science · 2026Review
- Advances in the dissection of Anopheles-Plasmodium interactions.PLoS pathogens · 2025Review
- Identification of a dengue 2 virus envelope protein receptor inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Response of the mosquito immune system and symbiotic bacteria to pathogen infection.Parasites & vectors · 2024Review
- Mosquito Innate Immunity.Insects · 2018Review
- Serine protease-related proteins in the malaria mosquito, Anopheles gambiae.Insect biochemistry and molecular biology · 2017Article
- The transcriptome of the mosquito Aedes fluviatilis (Diptera: Culicidae), and transcriptional changes associated with its native Wolbachia infection.BMC genomics · 2017Article
- Gene expression changes in the salivary glands of Anopheles coluzzii elicited by Plasmodium berghei infection.Parasites & vectors · 2015Article
- An overview of malaria transmission from the perspective of Amazon Anopheles vectors.Memorias do Instituto Oswaldo Cruz · 2015Review
- The Plasmodium bottleneck: malaria parasite losses in the mosquito vector.Memorias do Instituto Oswaldo Cruz · 2014Article
- Anopheles gambiae circumsporozoite protein-binding protein facilitates plasmodium infection of mosquito salivary glands.The Journal of infectious diseases · 2013Article
- Immunisation against a serine protease inhibitor reduces intensity of Plasmodium berghei infection in mosquitoes.International journal for parasitology · 2013Article
- A compendium of molecules involved in vector-pathogen interactions pertaining to malaria.Malaria journal · 2013Review
- No evidence for positive selection at two potential targets for malaria transmission-blocking vaccines in Anopheles gambiae s.s.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2013Article
- Article
- Biochemical characterization of Anopheles gambiae SRPN6, a malaria parasite invasion marker in mosquitoes.PloS one · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundPlasmodium parasites need to cross the midgut and salivary gland epithelia to complete their life cycle in the mosquito. However, our understanding of the molecular mechanism and the mosquito genes that participate in this process is still very limited. METHODOLOGY/PRINCIPAL
findingsWe identified an Anopheles gambiae epithelial serine protease (AgESP) that is constitutively expressed in the submicrovillar region of mosquito midgut epithelial cells and in the basal side of the salivary glands that is critical for Plasmodium parasites to cross these two epithelial barriers. AgESP silencing greatly reduces Plasmodium berghei and Plasmodium falciparum midgut invasion and prevents the transcriptional activation of gelsolin, a key regulator of actin remodeling and a reported Plasmodium agonist. AgESP expression is highly induced in midgut cells invaded by Plasmodium, suggesting that this protease also participates in the apoptotic response to invasion. In salivary gland epithelial cells, AgESP is localized on the basal side--the surface with which sporozoites interact. AgESP expression in the salivary gland is also induced in response to P. berghei and P. falciparum sporozoite invasion, and AgESP silencing significantly reduces the number of sporozoites that invade this organ.
conclusionOur findings indicate that AgESP is required for Plasmodium parasites to effectively traverse the midgut and salivary gland epithelial barriers. Plasmodium parasites need to modify the actin cytoskeleton of mosquito epithelial cells to successfully complete their life cycle in the mosquito and AgESP appears to be a major player in the regulation of this process.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.