ArticlePloS one2012
Glucose and fatty acid metabolism in a 3 tissue in-vitro model challenged with normo- and hyperglycaemia.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 55 citations in OpenAlex.
- Inter-organ communication shapes human metabolic tissue states and resolves anti-diabetic drug response modes in a six-tissue microphysiological system.bioRxiv : the preprint server for biology · 2026Article
- A pumpless liver-adipose model for studying metabolic dysfunction and drug responses.PloS one · 2026Article
- Plug and Play Multi-Organ Chips: Integrated µGaskets for the Facile and Reversible Connection of Individual Organ-on-Chip Modules.Micromachines · 2025Article
- 3D multicellular systems in disease modelling: From organoids to organ-on-chip.Frontiers in cell and developmental biology · 2023Review
- Studying metabolism with multi-organ chips: new tools for disease modelling, pharmacokinetics and pharmacodynamics.Open biology · 2022Review
- Evolution of Biochip Technology: A Review from Lab-on-a-Chip to Organ-on-a-Chip.Micromachines · 2020Review
- Use of dual-flow bioreactor to develop a simplified model of nervous-cardiovascular systems crosstalk: A preliminary assessment.PloS one · 2020Article
- Augmentation of Glucotoxicity, Oxidative Stress, Apoptosis and Mitochondrial Dysfunction in HepG2 Cells by Palmitic Acid.Nutrients · 2019Article
- Article
- Multiorgan Microphysiological Systems for Drug Development: Strategies, Advances, and Challenges.Advanced healthcare materials · 2018Review
- Incubation under fluid dynamic conditions markedly improves the structural preservation in vitro of explanted skeletal muscles.European journal of histochemistry : EJH · 2017Article
- Integrated gut/liver microphysiological systems elucidates inflammatory inter-tissue crosstalk.Biotechnology and bioengineering · 2017Article
- Tissue chips - innovative tools for drug development and disease modeling.Lab on a chip · 2017Review
- Building an experimental model of the human body with non-physiological parameters.Technology · 2017Article
- Functional Coupling of Human Microphysiology Systems: Intestine, Liver, Kidney Proximal Tubule, Blood-Brain Barrier and Skeletal Muscle.Scientific reports · 2017Article
- Emerging In Vitro Liver Technologies for Drug Metabolism and Inter-Organ Interactions.Tissue engineering. Part B, Reviews · 2016Review
- Mimicking the Kidney: A Key Role in Organ-on-Chip Development.Micromachines · 2016Review
- Multi-Organ toxicity demonstration in a functional human in vitro system composed of four organs.Scientific reports · 2016Article
- The hepatocyte proteome in organotypic rat liver models and the influence of the local microenvironment.Proteome science · 2016Article
- Microengineered cell and tissue systems for drug screening and toxicology applications: Evolution ofTechnology · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nutrient balance in the human body is maintained through systemic signaling between different cells and tissues. Breaking down this circuitry to its most basic elements and reconstructing the metabolic network in-vitro provides a systematic method to gain a better understanding of how cross-talk between the organs contributes to the whole body metabolic profile and of the specific role of each different cell type. To this end, a 3-way connected culture of hepatocytes, adipose tissue and endothelial cells representing a simplified model of energetic substrate metabolism in the visceral region was developed. The 3-way culture was shown to maintain glucose and fatty acid homeostasis in-vitro. Subsequently it was challenged with insulin and high glucose concentrations to simulate hyperglycaemia. The aim was to study the capacity of the 3-way culture to maintain or restore normal circulating glucose concentrations in response to insulin and to investigate the effects these conditions on other metabolites involved in glucose and lipid metabolism. The results show that the system's metabolic profile changes dramatically in the presence of high concentrations of glucose, and that these changes are modulated by the presence of insulin. Furthermore, we observed an increase in E-selectin levels in hyperglycaemic conditions and increased IL-6 concentrations in insulin-free-hyperglycaemic conditions, indicating, respectively, endothelial injury and proinflammatory stress in the challenged 3-way system.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.