ArticlePloS one2012
In situ monitoring of intracellular glucose and glutamine in CHO cell culture.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 50 citations in OpenAlex.
- Development of a cell-subculture bell (C-Bell) device using low-cost RGB color sensors.Scientific reports · 2026Article
- Fluorescence sensing of glucose at nanomolar levels using an imprinted copolymer embedded with carbon quantum dots.RSC advances · 2025Article
- Ensemble quantitation of absolute metabolite concentrations in T cells reveals conserved features of immunometabolism.bioRxiv : the preprint server for biology · 2025Article
- Recent advances in screening amino acid overproducers.Engineering microbiology · 2023Review
- Article
- Reprogramming of host glutamine metabolism during Chlamydia trachomatis infection and its key role in peptidoglycan synthesis.Nature microbiology · 2020Article
- Effective glucose metabolism maintains low intracellular glucose in airway epithelial cells after exposure to hyperglycemia.American journal of physiology. Cell physiology · 2019Article
- A One-Step Extraction and Luminescence Assay for Quantifying Glucose and ATP Levels in Cultured HepG2 Cells.International journal of molecular sciences · 2018Article
- Single Cell "Glucose Nanosensor" Verifies Elevated Glucose Levels in Individual Cancer Cells.Nano letters · 2016Article
- Ribosomal protein S6 phosphorylation is controlled by TOR and modulated by PKA in Candida albicans.Molecular microbiology · 2015Article
- A critique of methods for temperature imaging in single cells.Nature methods · 2014Article
- FIBS-enabled noninvasive metabolic profiling.Journal of visualized experiments : JoVE · 2014Article
- Mitochondrial metabolism of glucose and glutamine is required for intracellular growth of Toxoplasma gondii.Cell host & microbe · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The development of processes to produce biopharmaceuticals industrially is still largely empirical and relies on optimizing both medium formulation and cell line in a product-specific manner. Current small-scale (well plate-based) process development methods cannot provide sufficient sample volume for analysis, to obtain information on nutrient utilization which can be problematic when processes are scaled to industrial fermenters. We envision a platform where essential metabolites can be monitored non-invasively and in real time in an ultra-low volume assay in order to provide additional information on cellular metabolism in high throughput screens. Towards this end, we have developed a model system of Chinese Hamster Ovary cells stably expressing protein-based biosensors for glucose and glutamine. Herein, we demonstrate that these can accurately reflect changing intracellular metabolite concentrations in vivo during batch and fed-batch culture of CHO cells. The ability to monitor intracellular depletion of essential nutrients in high throughput will allow rapid development of improved bioprocesses.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.