ArticleMolecular vision2012
Microarray analysis of gene expression in West Nile virus-infected human retinal pigment epithelium.
Article in Molecular vision, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.
- Determination of system level alterations in host transcriptome due to Zika virus (ZIKV) Infection in retinal pigment epithelium.Scientific reports · 2018Pooled it
- Evolution-guided prioritization identifies a tissue-specific phosphorylation switch on herpes simplex virus 1 UL7 regulating viral replication and pathogenicity.Journal of virology · 2026Article
- Insight into ocular complications of West Nile Virus: A case report of chorioretinal scarring.IDCases · 2024Article
- Interleukins, Chemokines, and Tumor Necrosis Factor Superfamily Ligands in the Pathogenesis of West Nile Virus Infection.Viruses · 2023Review
- Signaling events evoked by domain III of envelop glycoprotein of tick-borne encephalitis virus and West Nile virus in human brain microvascular endothelial cells.Scientific reports · 2022Article
- Zika Virus Infection of Human Iris Pigment Epithelial Cells.Frontiers in immunology · 2021Article
- Ocular Manifestations of West Nile Virus.Vaccines · 2020Review
- Basic insights into Zika virus infection of neuroglial and brain endothelial cells.The Journal of general virology · 2020Article
- Inflammatory responses to a pathogenic West Nile virus strain.BMC infectious diseases · 2019Article
- AAVProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Network meta-analysis correlates with analysis of merged independent transcriptome expression data.BMC bioinformatics · 2019Article
- Specific correlation between the major chromosome 10q26 haplotype conferring risk for age-related macular degeneration and the expression ofMolecular vision · 2017Article
- Molecular Responses of Human Retinal Cells to Infection with Dengue Virus.Mediators of inflammation · 2017Article
- Transcriptomic Analyses Reveal Differential Gene Expression of Immune and Cell Death Pathways in the Brains of Mice Infected with West Nile Virus and Chikungunya Virus.Frontiers in microbiology · 2017Article
- Cullin4 Is Pro-Viral during West Nile Virus Infection of Culex Mosquitoes.PLoS pathogens · 2015Article
- Human organic anion transporting polypeptide 1A2 (OATP1A2) mediates cellular uptake of all-trans-retinol in human retinal pigmented epithelial cells.British journal of pharmacology · 2015Article
- West Nile Virus Challenge Alters the Transcription Profiles of Innate Immune Genes in Rabbit Peripheral Blood Mononuclear Cells.Frontiers in veterinary science · 2015Article
- Article
- Multiplexed digital mRNA profiling of the inflammatory response in the West Nile Swiss Webster mouse model.PLoS neglected tropical diseases · 2014Article
- A role for human brain pericytes in neuroinflammation.Journal of neuroinflammation · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo identify key genes differentially expressed in the human retinal pigment epithelium (hRPE) following low-level West Nile virus (WNV) infection.
methodsPrimary hRPE and retinal pigment epithelium cell line (ARPE-19) cells were infected with WNV (multiplicity of infection 1). RNA extracted from mock-infected and WNV-infected cells was assessed for differential expression of genes using Affymetrix microarray. Quantitative real-time PCR analysis of 23 genes was used to validate the microarray results.
resultsFunctional annotation clustering of the microarray data showed that gene clusters involved in immune and antiviral responses ranked highly, involving genes such as chemokine (C-C motif) ligand 2 (CCL2), chemokine (C-C motif) ligand 5 (CCL5), chemokine (C-X-C motif) ligand 10 (CXCL10), and toll like receptor 3 (TLR3). In conjunction with the quantitative real-time PCR analysis, other novel genes regulated by WNV infection included indoleamine 2,3-dioxygenase (IDO1), genes involved in the transforming growth factor-β pathway (bone morphogenetic protein and activin membrane-bound inhibitor homolog [BAMBI] and activating transcription factor 3 [ATF3]), and genes involved in apoptosis (tumor necrosis factor receptor superfamily, member 10d [TNFRSF10D]). WNV-infected RPE did not produce any interferon-γ, suggesting that IDO1 is induced by other soluble factors, by the virus alone, or both.
conclusionsLow-level WNV infection of hRPE cells induced expression of genes that are typically associated with the host cell response to virus infection. We also identified other genes, including IDO1 and BAMBI, that may influence the RPE and therefore outer blood-retinal barrier integrity during ocular infection and inflammation, or are associated with degeneration, as seen for example in aging.
Indexed as
Identifiers
22509103PMC3324360W2108730563What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.