ArticleJournal of applied crystallography2012
High-throughput biological small-angle X-ray scattering with a robotically loaded capillary cell.
Article in Journal of applied crystallography, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
43 citing papers in PubMed, 63 citations in OpenAlex.
- Coenzyme A is a redox sensing cofactor for malic enzyme 2 regulating oxidative stress and mitochondrial metabolism.bioRxiv : the preprint server for biology · 2026Article
- Visualizing and accessing correlated SAXS data sets with Similarity Maps and Simple Scattering web resources.Methods in enzymology · 2023Article
- Structure of Rift Valley Fever Virus RNA-Dependent RNA Polymerase.Journal of virology · 2022Article
- Nanocrystalline protein domains via salting-out.Acta crystallographica. Section F, Structural biology communications · 2021Article
- Tools for supporting solution scattering during the COVID-19 pandemic.Journal of synchrotron radiation · 2021Article
- High-pressure small-angle X-ray scattering cell for biological solutions and soft materials.Journal of applied crystallography · 2021Article
- Structure and function of the juxtamembrane GAF domain of potassium biosensor KdpD.Protein science : a publication of the Protein Society · 2020Article
- Solution scattering at the Life Science X-ray Scattering (LiX) beamline.Journal of synchrotron radiation · 2020Article
- Quantitative Measure of the Size Dispersity in Ultrasmall Fluorescent Organic-Inorganic Hybrid Core-Shell Silica Nanoparticles by Small-angle X-ray Scattering.Chemistry of materials : a publication of the American Chemical Society · 2019Article
- Smaller capillaries improve the small-angle X-ray scattering signal and sample consumption for biomacromolecular solutions.Journal of synchrotron radiation · 2018Article
- Crystal and solution structures of human protein-disulfide isomerase-like protein of the testis (PDILT) provide insight into its chaperone activity.The Journal of biological chemistry · 2018Article
- 7-Phenoxy-Substituted 3,4-Dihydro-2H-1,2,4-benzothiadiazine 1,1-Dioxides as Positive Allosteric Modulators of α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptors with Nanomolar Potency.Journal of medicinal chemistry · 2018Article
- Extended low-resolution structure of aeLife · 2017Article
- Designing and defining dynamic protein cage nanoassemblies in solution.Science advances · 2016Article
- Improved radiation dose efficiency in solution SAXS using a sheath flow sample environment.Acta crystallographica. Section D, Structural biology · 2016Article
- Structural and functional analysis of an anchorless fibronectin-binding protein FBPS from Gram-positive bacterium Streptococcus suis.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Full-length model of the human galectin-4 and insights into dynamics of inter-domain communication.Scientific reports · 2016Article
- SAXS studies of RNA: structures, dynamics, and interactions with partners.Wiley interdisciplinary reviews. RNA · 2016Review
- Quantifying radiation damage in biomolecular small-angle X-ray scattering.Journal of applied crystallography · 2016Article
- Allosteric Inhibition of Human Ribonucleotide Reductase by dATP Entails the Stabilization of a Hexamer.Biochemistry · 2016Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
With the rise in popularity of biological small-angle X-ray scattering (BioSAXS) measurements, synchrotron beamlines are confronted with an ever-increasing number of samples from a wide range of solution conditions. To meet these demands, an increasing number of beamlines worldwide have begun to provide automated liquid-handling systems for sample loading. This article presents an automated sample-loading system for BioSAXS beamlines, which combines single-channel disposable-tip pipetting with a vacuum-enclosed temperature-controlled capillary flow cell. The design incorporates an easily changeable capillary to reduce the incidence of X-ray window fouling and cross contamination. Both the robot-control and the data-processing systems are written in Python. The data-processing code, RAW, has been enhanced with several new features to form a user-friendly BioSAXS pipeline for the robot. The flow cell also supports efficient manual loading and sample recovery. An effective rinse protocol for the sample cell is developed and tested. Fluid dynamics within the sample capillary reveals a vortex ring pattern of circulation that redistributes radiation-damaged material. Radiation damage is most severe in the boundary layer near the capillary surface. At typical flow speeds, capillaries below 2 mm in diameter are beginning to enter the Stokes (creeping flow) regime in which mixing due to oscillation is limited. Analysis within this regime shows that single-pass exposure and multiple-pass exposure of a sample plug are functionally the same with regard to exposed volume when plug motion reversal is slow. The robot was tested on three different beamlines at the Cornell High-Energy Synchrotron Source, with a variety of detectors and beam characteristics, and it has been used successfully in several published studies as well as in two introductory short courses on basic BioSAXS methods.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.