ArticleLab on a chip2012
Label-free quantitation of peptide release from neurons in a microfluidic device with mass spectrometry imaging.
Article in Lab on a chip, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Development of a C-reactive protein quantification method based on flow rate measurement of an ink solution pushed out by oxygen gas generated by catalase reaction.Mikrochimica acta · 2023Article
- Development and validation of an automated microfluidic perfusion platform for parallelized screening of compounds in vitro.Basic & clinical pharmacology & toxicology · 2023Article
- Recent advances in mass spectrometry analysis of neuropeptides.Mass spectrometry reviews · 2023Review
- Cell Analysis on Microfluidics Combined with Mass Spectrometry.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021Review
- Neuropeptidomics: Improvements in Mass Spectrometry Imaging Analysis and Recent Advancements.Current protein & peptide science · 2021Review
- Adsorption and Absorption of Collagen Peptides to Polydimethlysiloxane and Its Influence on Platelet Adhesion Flow Assays.Micromachines · 2020Article
- A microfluidic platform for continuous monitoring of dopamine homeostasis in dopaminergic cells.Microsystems & nanoengineering · 2019Article
- Integrating Mass Spectrometry with Microphysiological Systems for Improved Neurochemical Studies.Microphysiological systems · 2018Article
- A neuron-in-capillary platform for facile collection and mass spectrometric characterization of a secreted neuropeptide.Scientific reports · 2016Article
- Peptidomics for the discovery and characterization of neuropeptides and hormones.Trends in pharmacological sciences · 2015Review
- Mass spectrometry-based characterization of endogenous peptides and metabolites in small volume samples.Biochimica et biophysica acta · 2015Review
- Microfluidics-to-mass spectrometry: a review of coupling methods and applications.Journal of chromatography. A · 2015Review
- Small-volume analysis of cell-cell signaling molecules in the brain.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2014Review
- Article
- Laminar stream of detergents for subcellular neurite damage in a microfluidic device: a simple tool for the study of neuroregeneration.Journal of neural engineering · 2013Article
- Microfluidic systems for studying neurotransmitters and neurotransmission.Lab on a chip · 2013Review
- Micro total analysis systems: fundamental advances and applications in the laboratory, clinic, and field.Analytical chemistry · 2013Review
- Relative quantitation of neuropeptides over a thousand-fold concentration range.Journal of the American Society for Mass Spectrometry · 2012Article
- New perspectives on neuronal development via microfluidic environments.Trends in neurosciences · 2012Review
- Over a century of neuron culture: from the hanging drop to microfluidic devices.The Yale journal of biology and medicine · 2012Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Microfluidic technology allows the manipulation of mass-limited samples and when used with cultured cells, enables control of the extracellular microenvironment, making it well suited for studying neurons and their response to environmental perturbations. While matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) provides for off-line coupling to microfluidic devices for characterizing small-volume extracellular releasates, performing quantitative studies with MALDI is challenging. Here we describe a label-free absolute quantitation approach for microfluidic devices. We optimize device fabrication to prevent analyte losses before measurement and then incorporate a substrate that collects the analytes as they flow through a collection channel. Following collection, the channel is interrogated using MS imaging. Rather than quantifying the sample present via MS peak height, the length of the channel containing appreciable analyte signal is used as a measure of analyte amount. A linear relationship between peptide amount and band length is suggested by modeling the adsorption process and this relationship is validated using two neuropeptides, acidic peptide (AP) and α-bag cell peptide [1-9] (αBCP). The variance of length measurement, defined as the ratio of standard error to mean value, is as low as 3% between devices. The limit of detection (LOD) of our system is 600 fmol for AP and 400 fmol for αBCP. Using appropriate calibrations, we determined that an individual Aplysia bag cell neuron secretes 0.15 ± 0.03 pmol of AP and 0.13 ± 0.06 pmol of αBCP after being stimulated with elevated KCl. This quantitation approach is robust, does not require labeling, and is well suited for miniaturized off-line characterization from microfluidic devices.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.