ArticleJournal of visualized experiments : JoVE2012
Wholemount immunohistochemistry for revealing complex brain topography.
Article in Journal of visualized experiments : JoVE, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 17 citations in OpenAlex.
- Article
- Shaping Diversity Into the Brain's Form and Function.Frontiers in neural circuits · 2018Review
- Using a Whole-mount Immunohistochemical Method to Study the Innervation of the Biliary Tract in Suncus murinus.Journal of visualized experiments : JoVE · 2017Article
- Lighting up Neuroanatomy.Frontiers in neuroscience · 2016Article
- Maternal immune activation produces cerebellar hyperplasia and alterations in motor and social behaviors in male and female mice.Cerebellum (London, England) · 2015Article
- Genetic manipulation of cerebellar granule neurons in vitro and in vivo to study neuronal morphology and migration.Journal of visualized experiments : JoVE · 2014Article
- Establishment of topographic circuit zones in the cerebellum of scrambler mutant mice.Frontiers in neural circuits · 2013Article
- Postnatal development of cerebellar zones revealed by neurofilament heavy chain protein expression.Frontiers in neuroanatomy · 2013Article
- Architecture and development of olivocerebellar circuit topography.Frontiers in neural circuits · 2012Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The repeated and well-understood cellular architecture of the cerebellum make it an ideal model system for exploring brain topography. Underlying its relatively uniform cytoarchitecture is a complex array of parasagittal domains of gene and protein expression. The molecular compartmentalization of the cerebellum is mirrored by the anatomical and functional organization of afferent fibers. To fully appreciate the complexity of cerebellar organization we previously refined a wholemount staining approach for high throughput analysis of patterning defects in the mouse cerebellum. This protocol describes in detail the reagents, tools, and practical steps that are useful to successfully reveal protein expression patterns in the adult mouse cerebellum by using wholemount immunostaining. The steps highlighted here demonstrate the utility of this method using the expression of zebrinII/aldolaseC as an example of how the fine topography of the brain can be revealed in its native three-dimensional conformation. Also described are adaptations to the protocol that allow for the visualization of protein expression in afferent projections and large cerebella for comparative studies of molecular topography. To illustrate these applications, data from afferent staining of the rat cerebellum are included.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.