Trial reportBlood transfusion = Trasfusione del sangue2012
Temporal sequence of major biochemical events during blood bank storage of packed red blood cells.
Trial report in Blood transfusion = Trasfusione del sangue, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 76 citations in OpenAlex.
- Trial
- Storing red blood cells with vitamin C and N-acetylcysteine prevents oxidative stress-related lesions: a metabolomics overview.Blood transfusion = Trasfusione del sangue · 2014Trial
- TheHeliyon · 2023Article
- Label-free testing strategy to evaluate packed red blood cell quality before transfusion to leukemia patients.Scientific reports · 2022Article
- Improving packed red blood cell storage with a high-viscosity buffered storage solution.Surgery · 2022Article
- Sex-dependent membranopathy in stored human red blood cells.Haematologica · 2021Article
- Do We Store Packed Red Blood Cells under "Quasi-Diabetic" Conditions?Biomolecules · 2021Review
- Article
- Glutaraldehyde - A Subtle Tool in the Investigation of Healthy and Pathologic Red Blood Cells.Frontiers in physiology · 2019Article
- Optofluidic laser speckle image decorrelation analysis for the assessment of red blood cell storage.PloS one · 2019Article
- Red blood cell storage lesion: causes and potential clinical consequences.Blood transfusion = Trasfusione del sangue · 2019Review
- Morphology, membrane nanostructure and stiffness for quality assessment of packed red blood cells.Scientific reports · 2017Article
- Acetylcholinesterase provides new insights into red blood cell ageing in vivo and in vitro.Blood transfusion = Trasfusione del sangue · 2017Article
- Specific Etiologies Associated With the Multiple Organ Dysfunction Syndrome in Children: Part 2.Pediatric critical care medicine : a journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies · 2017Review
- l-carnitine as a Potential Additive in Blood Storage Solutions: A Study on Erythrocytes.Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion · 2016Article
- Metabolic pathways that correlate with post-transfusion circulation of stored murine red blood cells.Haematologica · 2016Article
- Shape matters: the effect of red blood cell shape on perfusion of an artificial microvascular network.Transfusion · 2016Article
- Renitrosylation of banked human red blood cells improves deformability and reduces adhesivity.Transfusion · 2015Article
- Washing stored red blood cells in an albumin solution improves their morphologic and hemorheologic properties.Transfusion · 2015Article
- Biochemical assessment of red blood cells during storage by (1)H nuclear magnetic resonance spectroscopy. Identification of a biomarker of their level of protection against oxidative stress.Blood transfusion = Trasfusione del sangue · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundWe used sensitive spectroscopic techniques to measure changes in Band 3 oligomeric state during storage of packed red blood cells (RBC); these changes were compared to metabolic changes, RBC morphology, cholesterol and membrane protein loss, phospholipid reorganisation of the RBC membrane, and peroxidation of membrane lipid. The aim of the study was to temporally sequence major biochemical events occurring during cold storage, in order to determine which changes may underlie the structural defects in stored RBC. MATERIALS AND
methodsFifteen RBC units were collected from normal volunteers and stored under standard blood bank conditions; both metabolic changes and lipid parameters were measured by multiple novel assays including a new mass spectrometric measurement of isoprostane (lipid peroxidation) and flow cytometric assessment of CD47 expression. Band 3 oligomeric state was assessed by time-resolved phosphorescence anisotropy, and RBC morphology by microscopy of glutaraldehyde-fixed RBC.
resultsExtracellular pH decreased and extracellular potassium increased rapidly during cold storage. Band 3 on the RBC membrane aggregated into large oligomers early in the storage period and coincident with changes in RBC morphology. Membrane lipid changes, including loss of unesterified cholesterol, lipid peroxidation and expression of CD47, also changed early during the storage period. In contrast loss of acetylcholinesterase activity and haemolysis of RBC occurred late during storage. DISCUSSION: Our results demonstrate that changes in the macromolecular organisation of membrane proteins on the RBC occur early in storage and suggest that lipid peroxidation and/or oxidative damage to the membrane are responsible for irreversible morphological changes and loss of function during red cell storage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.