ArticleFoodborne pathogens and disease2012
Standardization and international multicenter validation of a PulseNet pulsed-field gel electrophoresis protocol for subtyping Shigella flexneri isolates.
Article in Foodborne pathogens and disease, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Article
- AnalyzingInfection and drug resistance · 2025Article
- Characterization of Shigella flexneri Serotype 6 Strains Isolated from Bangladesh and Identification of a New Phylogenetic Cluster.Journal of bacteriology · 2023Article
- Article
- PulseNet Latin America and the Caribbean Network: Present and Future.Foodborne pathogens and disease · 2019Article
- PulseNet: Entering the Age of Next-Generation Sequencing.Foodborne pathogens and disease · 2019Article
- PCR-Based Method for Shigella flexneri Serotyping: International Multicenter Validation.Journal of clinical microbiology · 2019Article
- PulseNet International: Vision for the implementation of whole genome sequencing (WGS) for global food-borne disease surveillance.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2017Review
- Molecular characterization and analysis of high-level multidrug-resistance of Shigella flexneri serotype 4s strains from China.Scientific reports · 2016Article
- Isolation and Comparative Genomic Analysis of T1-Like Shigella Bacteriophage pSf-2.Current microbiology · 2016Article
- Resistance Pattern and Molecular Characterization of Enterotoxigenic Escherichia coli (ETEC) Strains Isolated in Bangladesh.PloS one · 2016Article
- Article
- Article
- Laboratory-based prospective surveillance for community outbreaks of Shigella spp. in Argentina.PLoS neglected tropical diseases · 2013Article
Corrections and comments
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Authors and funding
17 authors.
Funding
Abstract
Shigella flexneri is one of the agents most frequently linked to diarrheal illness in developing countries and often causes outbreaks in settings with poor hygiene or sanitary conditions. Travel is one of the means by which S. flexneri can be imported into developed countries, where this pathogen is not commonly seen. A robust and discriminatory subtyping method is needed for the surveillance of S. flexneri locally and regionally, and to aid in the detection and investigation of outbreaks. The PulseNet International network utilizes standardized pulsed-field gel electrophoresis (PFGE) protocols to carry out laboratory-based surveillance of foodborne pathogens in combination with epidemiologic data. A multicenter validation was carried out in nine PulseNet laboratories located in North and South America, Europe, and Asia, and it demonstrated that a new protocol is highly robust and reproducible for subtyping of S. flexneri. This protocol, already approved for PulseNet laboratories, applies NotI and XbaI as primary and secondary restriction enzymes, respectively, under electrophoresis conditions of initial switch time of 5 s to final switch time of 35 s, at 6 volts/cm.
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