ArticlePloS one2012
Transcriptional regulation of opaR, qrr2-4 and aphA by the master quorum-sensing regulator OpaR in Vibrio parahaemolyticus.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 95 citations in OpenAlex.
- Toxin Secretion Systems Used by Marine Pathogenic Bacteria: A Review.Journal of fish diseases · 2026Review
- The Role of Phytosterol Derivatives in Inhibiting LuxS-Mediated Quorum Sensing and Biofilm Formation in Vibrio parahaemolyticus.Molecular biotechnology · 2026Article
- Functional divergence and regulatory network of the type VI secretion system inJournal of bacteriology · 2026Review
- Article
- AphA-dependent c-di-GMP production inJournal of bacteriology · 2025Article
- Role of Small Non-Coding RNA in Gram-Negative Bacteria: New Insights and Comprehensive Review of Mechanisms, Functions, and Potential Applications.Molecular biotechnology · 2025Review
- Investigating c-di-GMP Signaling in Vibrio parahaemolyticus: Biological Effects and Mechanisms of Regulation.Current microbiology · 2025Review
- Integrons are anti-phage defence libraries in Vibrio parahaemolyticus.Nature microbiology · 2025Article
- Quorum sensing regulates virulence factors in the coral pathogenApplied and environmental microbiology · 2025Article
- AcsS Negatively Regulates the Transcription of type VI Secretion System 2 Genes in Vibrio parahaemolyticus.Current microbiology · 2024Article
- IcmF2 of the type VI secretion system 2 plays a role in biofilm formation of Vibrio parahaemolyticus.Archives of microbiology · 2024Article
- Evaluation of Therapeutic Efficiency of Stylicin against Vibrio parahaemolyticus Infection in Shrimp Penaeus vannamei through Comparative Proteomic Approach.Probiotics and antimicrobial proteins · 2024Article
- ArcB orchestrates the quorum-sensing system to regulate type III secretion system 1 inGut microbes · 2023Article
- Transcriptomic Profiles of Vibrio parahaemolyticus During Biofilm Formation.Current microbiology · 2023Article
- OpaR Exerts a Dynamic Control over c-di-GMP Homeostasis andMicrobiology spectrum · 2023Article
- Heterologous expression of quorum sensing transcriptional regulator LitR and its function in virulence-related gene regulation in foodborne pathogen Aeromonas hydrophila.Molecular biology reports · 2023Article
- QsvR and OpaR coordinately repress biofilm formation byFrontiers in microbiology · 2023Article
- Quorum sensing and QsvR tightly control the transcription ofFrontiers in microbiology · 2023Article
- Computationally Designed Anti-LuxP DNA Aptamer Suppressed Flagellar Assembly- and Quorum Sensing-Related Gene Expression inBiology · 2022Article
- Gene Regulatory Network of the Noncoding RNAMicroorganisms · 2022Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundVibrio parahaemolyticus is a leading cause of infectious diarrhea and enterogastritis via the fecal-oral route. V. harveyi is a pathogen of fishes and invertebrates, and has been used as a model for quorum sensing (QS) studies. LuxR is the master QS regulator (MQSR) of V. harveyi, and LuxR-dependent expression of its own gene, qrr2-4 and aphA have been established in V. harveyi. Molecular regulation of target genes by the V. parahaemolyticus MQSR OpaR is still poorly understood. METHODOLOGY/PRINCIPAL
findingsThe bioinformatics analysis indicated that V. parahaemolyticus OpaR, V. harveyi LuxR, V. vulnificu SmcR, and V. alginolyticus ValR were extremely conserved, and that these four MQSRs appeared to recognize the same conserved cis-acting signals, which was represented by the consensus constructs manifesting as a position frequency matrix and as a 20 bp box, within their target promoters. The MQSR box-like sequences were found within the upstream DNA regions of opaR, qrr2-4 and aphA in V. parahaemolyticus, and the direct transcriptional regulation of these target genes by OpaR were further confirmed by multiple biochemical experiments including primer extension assay, gel mobility shift assay, and DNase I footprinting analysis. Translation and transcription starts, core promoter elements for sigma factor recognition, Shine-Dalgarno sequences for ribosome recognition, and OpaR-binding sites were determined for the five target genes of OpaR, which gave a structural map of the OpaR-dependent promoters. Further computational promoter analysis indicated the above regulatory circuits were shared by several other closely related Vibrios but with slight exceptions. CONCLUSIONS/SIGNIFICANCE: This study gave a comprehensive computational and characterization of the direct transcriptional regulation of five target genes, opaR, qrr2-4 and ahpA, by OpaR in V. parahaemolyticus. These characterized regulatory circuits were conserved in V. harveyi and V. parahaemolyticus.
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