ArticlePloS one2012
Polygenic sex determination system in zebrafish.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 100 papers, 1 of them a synthesis that pooled it.
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Who cites it
100 citing papers in PubMed, 1 synthesis or guideline pooled it, 264 citations in OpenAlex.
- Sex-specific differences in zebrafish brains.Biology of sex differences · 2022Pooled it
- Zebrafish relatives as models for functional comparative genetics and genomics.Nature reviews. Genetics · 2026Review
- A lifespan atlas of the killifish testis defines a pronounced mid-age remodeling shift during testicular aging.BMC biology · 2026Article
- Environmental sex determination in the cyst nematode Globodera pallida defaults to male development.BMC biology · 2026Article
- Sex-Linked Differentiation in Commercially Exploited Fishes: Rethinking Population Structure in Dynamic Marine Environments.Global change biology · 2025Article
- Transposable Element-Mediated Cis-Regulation Drives the Evolution of dmrt1 as a Candidate Master Sex-Determining Gene in Black Carp.Molecular biology and evolution · 2025Article
- Cytoskeleton-associated protein 4 affects podocyte cytoskeleton dynamics in diabetic kidney disease.JCI insight · 2025Article
- Transcriptome analysis reveals the sex-switching mechanism of juvenile hermaphroditism in silver pomfret (Pampus argenteus).Biology of sex differences · 2025Article
- Identification of sex-biased MiRNA markers informative of heat-past events.BMC genomics · 2025Article
- Disrupting Amh and androgen signaling reveals their distinct roles in zebrafish gonadal differentiation and gametogenesis.Communications biology · 2025Article
- Evidence for Variation in the Genetic Basis of Sex Determination in Brook Stickleback (Ecology and evolution · 2025Article
- Lampbrush chromosomes of Danio rerio.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Article
- Sexual dimorphism in the DNA methylation pattern after immune stimulation in the zebrafish (Frontiers in cell and developmental biology · 2025Article
- Investigation of sex determination in starry flounder (Zoological research · 2024Article
- Adamts9 is required for the development of primary ovarian follicles and maintenance of female sex in zebrafish†.Biology of reproduction · 2024Article
- CRISPR/Cas9 Technology for Enhancing Desirable Traits of Fish Species in Aquaculture.International journal of molecular sciences · 2024Review
- Developmental Delay and Male-Biased Sex Ratio inGenes · 2024Article
- Effects of exogenous steroid hormones on growth, body color, and gonadal development in the Opsariichthys bidens.Fish physiology and biochemistry · 2024Article
- Macrophage activation drives ovarian failure and masculinization in zebrafish.Science advances · 2023Article
- Genetic evidence for differential functions of figla and nobox in zebrafish ovarian differentiation and folliculogenesis.Communications biology · 2023Article
40 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDespite the popularity of zebrafish as a research model, its sex determination (SD) mechanism is still unknown. Most cytogenetic studies failed to find dimorphic sex chromosomes and no primary sex determining switch has been identified even though the assembly of zebrafish genome sequence is near to completion and a high resolution genetic map is available. Recent publications suggest that environmental factors within the natural range have minimal impact on sex ratios of zebrafish populations. The primary aim of this study is to find out more about how sex is determined in zebrafish. METHODOLOGY/PRINCIPAL
findingsUsing classical breeding experiments, we found that sex ratios across families were wide ranging (4.8% to 97.3% males). On the other hand, repeated single pair crossings produced broods of very similar sex ratios, indicating that parental genotypes have a role in the sex ratio of the offspring. Variation among family sex ratios was reduced after selection for breeding pairs with predominantly male or female offspring, another indication that zebrafish sex is regulated genetically. Further examinations by a PCR-based "blind assay" and array comparative genomic hybridization both failed to find universal sex-linked differences between the male and female genomes. Together with the ability to increase the sex bias of lines by selective breeding, these data suggest that zebrafish is unlikely to utilize a chromosomal sex determination (CSD) system. CONCLUSIONS/SIGNIFICANCE: Taken together, our study suggests that zebrafish sex is genetically determined with limited, secondary influences from the environment. As we have not found any sign for CSD in the species, we propose that the zebrafish has a polygenic sex determination system.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.