ArticleThe Journal of biological chemistry2012
Unexpected link between lipooligosaccharide biosynthesis and surface protein release in Mycobacterium marinum.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 49 citations in OpenAlex.
- Comparative genomics and transcriptomics of rough and smooth Mycobacterium marinum focusing on ESX-1, ESX-6 and LOS genes.Scientific reports · 2026Article
- MmpL12 transports lipooligosaccharides and impacts virulence inMicrobiology (Reading, England) · 2025Article
- Conserved mycobacterial sRNA B11 regulates lipooligosaccharide synthesis at posttranscriptional level inmLife · 2025Article
- A glycosylated lipooctapeptide promotes uptake and growth of Mycobacterium abscessus in the host.Nature communications · 2025Article
- Type VII secretion system gene mutations driving global mycobacterium tuberculosis transmission revealed by whole genomic sequence.Frontiers in cellular and infection microbiology · 2025Article
- Insights into the ancestry evolution of theNAR genomics and bioinformatics · 2021Article
- Population genomics provides insights into the evolution and adaptation to humans of the waterborne pathogen Mycobacterium kansasii.Nature communications · 2021Article
- A Murine Model ofFrontiers in microbiology · 2021Article
- Conserved ESX-1 Substrates EspE and EspF Are Virulence Factors That Regulate Gene Expression.Infection and immunity · 2020Article
- Pulmonary non-tuberculous mycobacterial infections: current state and future management.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Review
- Role of theFrontiers in microbiology · 2018Article
- Severe inhibition of lipooligosaccharide synthesis induces TLR2-dependent elimination of Mycobacterium marinum from THP1-derived macrophages.Microbial cell factories · 2017Article
- Esx Systems and the Mycobacterial Cell Envelope: What's the Connection?Journal of bacteriology · 2017Review
- WhiB4 Regulates the PE/PPE Gene Family and is Essential for Virulence of Mycobacterium marinum.Scientific reports · 2017Article
- The Impact of Genome Region of Difference 4 (RD4) on Mycobacterial Virulence and BCG Efficacy.Frontiers in cellular and infection microbiology · 2017Article
- The Structure-Antimicrobial Activity Relationships of a Promising Class of the Compounds Containing the N-Arylpiperazine Scaffold.Molecules (Basel, Switzerland) · 2016Article
- The ESX-5 System of Pathogenic Mycobacteria Is Involved In Capsule Integrity and Virulence through Its Substrate PPE10.PLoS pathogens · 2016Article
- pks5-recombination-mediated surface remodelling in Mycobacterium tuberculosis emergence.Nature microbiology · 2016Article
- Neutrophils in host defense: new insights from zebrafish.Journal of leukocyte biology · 2015Review
- Genome-wide transposon mutagenesis indicates that Mycobacterium marinum customizes its virulence mechanisms for survival and replication in different hosts.Infection and immunity · 2015Article
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
Abstract
The mycobacterial cell envelope is characterized by the presence of a highly impermeable second membrane, which is composed of mycolic acids intercalated with different unusual free lipids, such as lipooligosaccharides (LOS). Transport across this cell envelope requires a dedicated secretion system for extracellular proteins, such as PE_PGRS proteins, which are specific mycobacterial proteins with polymorphic GC-rich sequence (PGRS). In this study, we set out to identify novel components involved in the secretion of PE_PGRS proteins by screening Mycobacterium marinum transposon mutants for secretion defects. Interestingly, most mutants were not affected in secretion but in the release of PE_PGRS proteins from the cell surface. These mutants had insertions in a gene cluster associated with LOS biosynthesis. Lipid analysis of these mutants revealed a role at different stages of LOS biosynthesis for 10 novel genes. Furthermore, we show that regulatory protein WhiB4 is involved in LOS biosynthesis. The absence of the most extended LOS molecule, i.e. LOS-IV, and a concomitant accumulation of LOS-III was already sufficient to reduce the release of PE_PGRS proteins from the mycobacterial cell surface. A similar effect was observed for major surface protein EspE. These results show that the attachment of surface proteins is strongly influenced by the glycolipid composition of the mycobacterial cell envelope. Finally, we tested the virulence of a LOS-IV-deficient mutant in our zebrafish embryo infection model. This mutant showed a marked increase in virulence as compared with the wild-type strain, suggesting that LOS-IV plays a role in the modulation of mycobacterial virulence.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.