ArticleJournal of bacteriology2012
The Pseudomonas aeruginosa global regulator VqsR directly inhibits QscR to control quorum-sensing and virulence gene expression.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Characterization of hypothetical proteins reveals function modules and virulence factors in Pseudomonas aeruginosa.Nature communications · 2026Article
- Engineered microorganisms unlock a new era of diterpenoid biomanufacturing: recent advances and future perspectives.Bioresources and bioprocessing · 2026Review
- Understanding the pathophysiology ofBurns & trauma · 2025Review
- The phage-encoded protein PIT2 impactsiScience · 2023Article
- The transcriptional regulators of virulence forGenes & diseases · 2023Review
- Frequency of quorum-sensing mutations inMicrobiology (Reading, England) · 2022Article
- Genetic and Transcriptomic Characteristics of RhlR-Dependent Quorum Sensing in Cystic Fibrosis Isolates of Pseudomonas aeruginosa.mSystems · 2022Article
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- Quantitative mapping of mRNA 3' ends in Pseudomonas aeruginosa reveals a pervasive role for premature 3' end formation in response to azithromycin.PLoS genetics · 2021Article
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- Multidrug Adaptive Resistance of Pseudomonas aeruginosa Swarming Cells.Antimicrobial agents and chemotherapy · 2020Article
- An integrated genomic regulatory network of virulence-related transcriptional factors in Pseudomonas aeruginosa.Nature communications · 2019Article
- RpoN-Dependent Direct Regulation of Quorum Sensing and the Type VI Secretion System in Pseudomonas aeruginosa PAO1.Journal of bacteriology · 2018Article
- Crystal structure of the N-terminal domain of VqsR from Pseudomonas aeruginosa at 2.1 Å resolution.Acta crystallographica. Section F, Structural biology communications · 2017Article
- Additive Effects of Quorum Sensing Anti-Activators onFrontiers in microbiology · 2017Article
- RpoN Modulates Carbapenem Tolerance in Pseudomonas aeruginosa through Pseudomonas Quinolone Signal and PqsE.Antimicrobial agents and chemotherapy · 2016Article
- A novel signal transduction pathway that modulates rhl quorum sensing and bacterial virulence in Pseudomonas aeruginosa.PLoS pathogens · 2014Article
- An evolving perspective on the Pseudomonas aeruginosa orphan quorum sensing regulator QscR.Frontiers in cellular and infection microbiology · 2014Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The opportunistic pathogen Pseudomonas aeruginosa has at least three quorum-sensing (QS) systems, including the acyl-homoserine lactone (acyl-HSL)-mediated las and rhl systems, as well as the 2-alkyl-4(1H)-quinolone (AHQ) signal-based system. A group of key regulators of these QS systems have been identified, such as qteE, vqsM, vqsR, and vfr. However, the underlying regulatory mechanisms of these QS systems are not yet fully understood. Here, using electrophoretic mobility shift assays, we demonstrated that VqsR indirectly regulates acyl-HSL systems but specifically binds to the qscR promoter region, which indicates that VqsR influences QS-controlled pathways through QscR. Through a dye-based DNase I footprint assay, we showed that VqsR interacts with an inverted repeat (IR) motif (TCGCCN(8)GGCGA, where N is any nucleotide) in the promoter region of qscR. A genome-wide search identified 50 other promoter regions carrying the same putative IR motif. The recombinant VqsR protein exists as a homodimer in solution. In addition, using a qscR-lux reporter assay and Northern blot hybridization, we found that the transcription level of qscR increased 4-fold in the vqsR deletion strain compared to the wild-type PAO1 strain, indicating vqsR as a negative regulator of qscR. Taken together, these findings provide new insights into the complex regulation network of QS systems in P. aeruginosa.
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