ArticleNucleic acids research2012
Interactions between BRCA2 and RAD51 for promoting homologous recombination in Leishmania infantum.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 39 citations in OpenAlex.
- Genome-wide mapping of replication stress response factors reveals the management of polycistronic transcription, replication initiation, and responses to replication stress in Leishmania.Nucleic acids research · 2026Article
- Modulation of TvRAD51 Recombinase inPathogens (Basel, Switzerland) · 2025Article
- Life in plastic, it's fantastic! HowFrontiers in cellular and infection microbiology · 2023Review
- The paradigm of intracellular parasite survival and drug resistance in leishmanial parasite through genome plasticity and epigenetics: Perception and future perspective.Frontiers in cellular and infection microbiology · 2023Review
- Plasmodium berghei Brca2 is required for normal development and differentiation in mice and mosquitoes.Parasites & vectors · 2022Article
- Divergent binding mode for a protozoan BRC repeat to RAD51.The Biochemical journal · 2022Article
- DNA Double-Strand Breaks: A Double-Edged Sword for Trypanosomatids.Frontiers in cell and developmental biology · 2021Review
- Conditional knockout of RAD51-related genes in Leishmania major reveals a critical role for homologous recombination during genome replication.PLoS genetics · 2020Article
- Evaluation of mechanisms that may generate DNA lesions triggering antigenic variation in African trypanosomes.PLoS pathogens · 2018Review
- Conservation and Variation in Strategies for DNA Replication of Kinetoplastid Nuclear Genomes.Current genomics · 2018Review
- Homologous Recombination in Protozoan Parasites and Recombinase Inhibitors.Frontiers in microbiology · 2017Review
- Roles for APRIN (PDS5B) in homologous recombination and in ovarian cancer prediction.Nucleic acids research · 2016Article
- Plasticity of theF1000Research · 2016Review
- Novel BRCA2-Interacting Protein, LIMD1, Is Essential for the Centrosome Localization of BRCA2 in Esophageal Cancer Cell.Oncology research · 2016Article
- Design, synthesis, and characterization of BRC4 mutants based on the crystal structure of BRC4-RAD51(191-220).Journal of molecular modeling · 2015Article
- Roles of Rad51 paralogs for promoting homologous recombination in Leishmania infantum.Nucleic acids research · 2015Article
- Formation of linear amplicons with inverted duplications in Leishmania requires the MRE11 nuclease.PLoS genetics · 2014Article
- RecO protein initiates DNA recombination and strand annealing through two alternative DNA binding mechanisms.The Journal of biological chemistry · 2014Article
- Genome-wide stochastic adaptive DNA amplification at direct and inverted DNA repeats in the parasite Leishmania.PLoS biology · 2014Article
- DNA repair pathways in trypanosomatids: from DNA repair to drug resistance.Microbiology and molecular biology reviews : MMBR · 2014Review
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
In most organisms, the primary function of homologous recombination (HR) is to allow genome protection by the faithful repair of DNA double-strand breaks. The vital step of HR is the search for sequence homology, mediated by the RAD51 recombinase, which is stimulated further by proteins mediators such as the tumor suppressor BRCA2. The biochemical interplay between RAD51 and BRCA2 is unknown in Leishmania or Trypanosoma. Here we show that the Leishmania infantum BRCA2 protein possesses several critical features important for the regulation of DNA recombination at the genetic and biochemical level. A BRCA2 null mutant, generated by gene disruption, displayed genomic instability and gene-targeting defects. Furthermore, cytological studies show that LiRAD51 can no longer localize to the nucleus in this mutant. The Leishmania RAD51 and BRCA2 interact together and the purified proteins bind single-strand DNA. Remarkably, LiBRCA2 is a recombination mediator that stimulates the invasion of a resected DNA double-strand break in an undamaged template by LiRAD51 to form a D-loop structure. Collectively, our data show that LiBRCA2 and LiRAD51 promote HR at the genetic and biochemical level in L. infantum, the causative agent of visceral leishmaniasis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.