ArticleThe EMBO journal2012
40S subunit dissociation and proteasome-dependent RNA degradation in nonfunctional 25S rRNA decay.
Article in The EMBO journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 58 citations in OpenAlex.
- Depurination of sarcin/ricin loop 25S rRNA is signaled through the small ribosomal subunit during translation.RNA (New York, N.Y.) · 2025Article
- RNA damage and its implications in genome stability.DNA repair · 2025Review
- The integrated stress response regulates 18S nonfunctional rRNA decay in mammals.Molecular cell · 2025Article
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- Phenomenological interpretations of the mechanism for the concentration-dependent positive effect of antibiotic lincomycin onApplied and environmental microbiology · 2023Article
- Co-Translational Quality Control Induced by Translational Arrest.Biomolecules · 2023Review
- Ubiquitin and Ubiquitin-Like Proteins and Domains in Ribosome Production and Function: Chance or Necessity?International journal of molecular sciences · 2021Review
- RNA Metabolism Guided by RNA Modifications: The Role of SMUG1 in rRNA Quality Control.Biomolecules · 2021Review
- Proteotoxic stress promotes entrapment of ribosomes and misfolded proteins in a shared cytosolic compartment.Nucleic acids research · 2020Article
- Quality controls induced by aberrant translation.Nucleic acids research · 2020Review
- Expanding Role of Ubiquitin in Translational Control.International journal of molecular sciences · 2020Review
- Review
- Review
- Pre-Ribosomal RNA Processing in Human Cells: From Mechanisms to Congenital Diseases.Biomolecules · 2018Review
- The RNA-binding protein Hfq is important for ribosome biogenesis and affects translation fidelity.The EMBO journal · 2018Article
- Noncoding RNA Surveillance: The Ends Justify the Means.Chemical reviews · 2018Review
- Ribosomal protein uS7/Rps5 serine-223 in protein kinase-mediated phosphorylation and ribosomal small subunit maturation.Scientific reports · 2018Article
- Crosstalk Between Mammalian Autophagy and the Ubiquitin-Proteasome System.Frontiers in cell and developmental biology · 2018Review
- Article
- Mechanisms of In Vivo Ribosome Maintenance Change in Response to Nutrient Signals.Molecular & cellular proteomics : MCP · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eukaryotic cells have quality control systems that eliminate nonfunctional rRNAs with deleterious mutations (nonfunctional rRNA decay, NRD). We have previously reported that 25S NRD requires an E3 ubiquitin ligase complex, which is involved in ribosomal ubiquitination. However, the degradation process of nonfunctional ribosomes has remained unknown. Here, using genetic screening, we identified two ubiquitin-binding complexes, the Cdc48-Npl4-Ufd1 complex (Cdc48 complex) and the proteasome, as the factors involved in 25S NRD. We show that the nonfunctional 60S subunit is dissociated from the 40S subunit in a Cdc48 complex-dependent manner, before it is attacked by the proteasome. When we examined the nonfunctional 60S subunits that accumulated under proteasome-depleted conditions, the majority of mutant 25S rRNAs retained their full length at a single-nucleotide resolution. This indicates that the proteasome is an essential factor triggering rRNA degradation. We further showed that ribosomal ubiquitination can be stimulated solely by the suppression of the proteasome, suggesting that ubiquitin-proteasome-dependent RNA degradation occurs in broader situations, including in general rRNA turnover.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.