ArticleApplied and environmental microbiology2012
In vitro functional and immunomodulatory properties of the Lactobacillus helveticus MIMLh5-Streptococcus salivarius ST3 association that are relevant to the development of a pharyngeal probiotic product.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- Probiotics in addition to antibiotics for the treatment of acute tonsillitis: a randomized, placebo-controlled study.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Trial
- Antibacterial and Immunomodulatory Effects ofMicroorganisms · 2026Article
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- Isolation and Characteristics of Extracellular Vesicles Produced by Probiotics: Yeast Saccharomyces boulardii CNCM I-745 and Bacterium Streptococcus salivarius K12.Probiotics and antimicrobial proteins · 2024Article
- A mix of chlorogenic and caffeic acid reduces C/EBPß and PPAR-γ1 levels and counteracts lipid accumulation in macrophages.European journal of nutrition · 2022Article
- Article
- Probiotics Modulate Mouse Gut Microbiota and Influence Intestinal Immune and Serotonergic Gene Expression in a Site-Specific Fashion.Frontiers in microbiology · 2021Article
- Surface Layer ofApplied and environmental microbiology · 2019Article
- A Novel Rhamnose-Rich Hetero-exopolysaccharide Isolated from Lactobacillus paracasei DG Activates THP-1 Human Monocytic Cells.Applied and environmental microbiology · 2017Article
- Mechanisms and therapeutic effectiveness of lactobacilli.Journal of clinical pathology · 2016Review
- The vaginal isolate Lactobacillus paracasei LPC-S01 (DSM 26760) is suitable for oral administration.Frontiers in microbiology · 2015Article
- Impact of kefir derived Lactobacillus kefiri on the mucosal immune response and gut microbiota.Journal of immunology research · 2015Article
- Do children's upper respiratory tract infections benefit from probiotics?BMC infectious diseases · 2014Review
- Lactobacillus helveticus MIMLh5-specific antibodies for detection of S-layer protein in Grana Padano protected-designation-of-origin cheese.Applied and environmental microbiology · 2014Article
- Otitis media among high-risk populations: can probiotics inhibit Streptococcus pneumoniae colonisation and the risk of disease?European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2013Review
- S-layer protein mediates the stimulatory effect of Lactobacillus helveticus MIMLh5 on innate immunity.Applied and environmental microbiology · 2013Article
- Beneficial effects of probiotics in upper respiratory tract infections and their mechanical actions to antagonize pathogens.Journal of applied microbiology · 2012Review
- Health-Promoting Properties of Lactobacillus helveticus.Frontiers in microbiology · 2012Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of proper bacterial strains as probiotics for the pharyngeal mucosa is a potential prophylactic strategy for upper respiratory tract infections. In this context, we characterized in vitro the functional and immunomodulatory properties of the strains Lactobacillus helveticus MIMLh5 and Streptococcus salivarius ST3 that were selected during previous investigations as promising pharyngeal probiotics. In this study, we demonstrated in vitro that strains MIMLh5 and ST3, alone and in combination, can efficiently adhere to pharyngeal epithelial cells, antagonize Streptococcus pyogenes, and modulate host innate immunity by inducing potentially protective effects. In particular, we found that the strains MIMLh5 and ST3 activate U937 human macrophages by significantly inducing the expression of the proinflammatory cytokine tumor necrosis factor alpha (TNF-α). Nonetheless, the induction of the anti-inflammatory interleukin-10 (IL-10) by MIMLh5 or ST3 was never lower than that of TNF-α, suggesting that these bacteria can potentially exert a regulatory rather than a proinflammatory effect. We also found that the strains MIMLh5 and ST3 induce cyclooxygenase 2 (COX-2) expression and demonstrated that toll-like receptor 2 (TLR-2) participates in the recognition of the strains MIMLh5 and ST3 by U937 cells. Finally, we observed that these microorganisms grow efficiently when cocultured in milk, suggesting that the preparation of a milk-based fermented product containing both MIMLh5 and ST3 can be a practical solution for the administration of these bacteria. In conclusion, we propose the combined use of L. helveticus MIMLh5 and S. salivarius ST3 for the preparation of novel products that display probiotic properties for the pharyngeal mucosa.
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