ArticleJournal of molecular biology2012
Dda helicase tightly couples translocation on single-stranded DNA to unwinding of duplex DNA: Dda is an optimally active helicase.
Article in Journal of molecular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Characterization and genomic analysis of vB_CoeS_P1, a novel marine bacteriophage, and definition of the new family Estoviridae.Archives of virology · 2026Article
- Bacterial RecD2 is a processive single-stranded DNA translocase with strand-switching capacity at DNA forks.Nucleic acids research · 2025Article
- The Role of SF1 and SF2 Helicases in Biotechnological Applications.Applied biochemistry and biotechnology · 2024Review
- Structural and functional insights into the interaction between the bacteriophage T4 DNA processing proteins gp32 and Dda.Nucleic acids research · 2024Article
- Subunit Communication within Dimeric SF1 DNA Helicases.Journal of molecular biology · 2024Article
- A real-time fluorescent gp32 probe-based assay for monitoring single-stranded DNA-dependent DNA processing enzymes.Biochemistry and biophysics reports · 2023Article
- Continuous millisecond conformational cycle of a DEAH box helicase reveals control of domain motions by atomic-scale transitions.Communications biology · 2023Article
- Monitoring helicase-catalyzed unwinding of multiple duplexes simultaneously.Methods in enzymology · 2022Article
- RNA helicases required for viral propagation in humans.The Enzymes · 2021Review
- Interplay between Position-Dependent Codon Usage Bias and Hydrogen Bonding at the 5' End of ORFeomes.mSystems · 2020Article
- Direct quantification of the translocation activities of Saccharomyces cerevisiae Pif1 helicase.Nucleic acids research · 2019Article
- Structure and function of Pif1 helicase.Biochemical Society transactions · 2017Review
- Modulation of Escherichia coli UvrD Single-Stranded DNA Translocation by DNA Base Composition.Biophysical journal · 2017Article
- A mechanistic study of helicases with magnetic traps.Protein science : a publication of the Protein Society · 2017Review
- Conformational selection and induced fit as a useful framework for molecular motor mechanisms.Biophysical chemistry · 2017Review
- High-temperature single-molecule kinetic analysis of thermophilic archaeal MCM helicases.Nucleic acids research · 2016Article
- Epigenetic and lncRNA regulation of cardiac pathophysiology.Biochimica et biophysica acta · 2016Review
- Yeast Helicase Pif1 Unwinds RNA:DNA Hybrids with Higher Processivity than DNA:DNA Duplexes.The Journal of biological chemistry · 2016Article
- Helicase processivity and not the unwinding velocity exhibits universal increase with force.Biophysical journal · 2015Article
- Insights into the mechanism of a G-quadruplex-unwinding DEAH-box helicase.Nucleic acids research · 2015Article
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Abstract
Helicases utilize the energy of ATP hydrolysis to unwind double-stranded DNA while translocating on the DNA. Mechanisms for melting the duplex have been characterized as active or passive, depending on whether the enzyme actively separates the base pairs or simply sequesters single-stranded DNA (ssDNA) that forms due to thermal fraying. Here, we show that Dda translocates unidirectionally on ssDNA at the same rate at which it unwinds double-stranded DNA in both ensemble and single-molecule experiments. Further, the unwinding rate is largely insensitive to the duplex stability and to the applied force. Thus, Dda transduces all of its translocase activity into DNA unwinding activity so that the rate of unwinding is limited by the rate of translocation and that the enzyme actively separates the duplex. Active and passive helicases have been characterized by dividing the velocity of DNA unwinding in base pairs per second (V(un)) by the velocity of translocation on ssDNA in nucleotides per second (V(trans)). If the resulting fraction is 0.25, then a helicase is considered to be at the lower end of the "active" range. In the case of Dda, the average DNA unwinding velocity was 257±42 bp/s, and the average translocation velocity was 267±15 nt/s. The V(un)/V(trans) value of 0.96 places Dda in a unique category of being an essentially "perfectly" active helicase.
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