ArticleJournal of biomedical optics2012
Multiphoton microscopy and microspectroscopy for diagnostics of inflammatory and neoplastic lung.
Article in Journal of biomedical optics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Real-time histological evaluation of gastrointestinal tissue using non-linear microscopy.Journal of clinical pathology · 2025Article
- Synchronously pumped Raman laser for simultaneous degenerate and nondegenerate two-photon microscopy.Biomedical optics express · 2021Article
- Hyperspectral multiphoton microscopy forOptica · 2020Article
- Compact portable multiphoton microscopy reveals histopathological hallmarks of unprocessed lung tumor tissue in real time.Translational biophotonics · 2020Article
- Characterization of a multicore fiber image guide for nonlinear endoscopic imaging using two-photon fluorescence and second-harmonic generation.Journal of biomedical optics · 2019Article
- Imaging of Murine Whole Lung Fibrosis by Large Scale 3D Microscopy aided by Tissue Optical Clearing.Scientific reports · 2018Article
- Real-time visualization of two-photon fluorescence lifetime imaging microscopy using a wavelength-tunable femtosecond pulsed laser.Biomedical optics express · 2018Article
- Noninvasive measurements of tissue perfusion in critical limb ischemia.Gefasschirurgie : Zeitschrift fur vaskulare und endovaskulare Chirurgie : Organ der Deutschen und der Osterreichischen Gesellschaft fur Gefasschirurgie unter Mitarbeit der Schweizerischen Gesellschaft fur Gefasschirurgie · 2018Review
- Rapid diagnosis and intraoperative margin assessment of human lung cancer with fluorescence lifetime imaging microscopy.BBA clinical · 2017Article
- Usefulness of Intravital Multiphoton Microscopy in Visualizing Study of Mouse Cochlea and Volume Changes in the Scala Media.Frontiers in neurology · 2017Article
- Direct comparison between confocal and multiphoton microscopy for rapid histopathological evaluation of unfixed human breast tissue.Journal of biomedical optics · 2016Article
- Autofluorescence multiphoton microscopy for visualization of tissue morphology and cellular dynamics in murine and human airways.Laboratory investigation; a journal of technical methods and pathology · 2016Article
- Virtual Hematoxylin and Eosin Transillumination Microscopy Using Epi-Fluorescence Imaging.PloS one · 2016Article
- Multiphoton gradient index endoscopy for evaluation of diseased human prostatic tissue ex vivo.Journal of biomedical optics · 2014Article
- Experimenting liver fibrosis diagnostic by two photon excitation microscopy and Bag-of-Features image classification.Scientific reports · 2014Article
- Molecular imaging for cancer diagnosis and surgery.Advanced drug delivery reviews · 2014Review
- Fluorescence-guided surgery with live molecular navigation--a new cutting edge.Nature reviews. Cancer · 2013Review
- Multimodal non-linear optical imaging for label-free differentiation of lung cancerous lesions from normal and desmoplastic tissues.Biomedical optics express · 2013Article
- Dual modality endomicroscope with optical zoom capability.Biomedical optics express · 2013Article
- In vivo imaging of unstained tissues using long gradient index lens multiphoton endoscopic systems.Biomedical optics express · 2012Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Limitations of current medical procedures for detecting early lung cancers inspire the need for new diagnostic imaging modalities for the direct microscopic visualization of lung nodules. Multiphoton microscopy (MPM) provides for subcellular resolution imaging of intrinsic fluorescence from unprocessed tissue with minimal optical attenuation and photodamage. We demonstrate that MPM detects morphological and spectral features of lung tissue and differentiates between normal, inflammatory and neoplastic lung. Ex vivo MPM imaging of intrinsic two-photon excited fluorescence was performed on mouse and canine neoplastic, inflammatory and tumor-free lung sites. Results showed that MPM detected microanatomical differences between tumor-free and neoplastic lung tissue similar to standard histopathology but without the need for tissue processing. Furthermore, inflammatory sites displayed a distinct red-shifted fluorescence compared to neoplasms in both mouse and canine lung, and adenocarcinomas displayed a less pronounced fluorescence emission in the 500 to 550 nm region compared to adenomas in mouse models of lung cancer. These spectral distinctions were also confirmed by two-photon excited fluorescence microspectroscopy. We demonstrate the feasibility of applying MPM imaging of intrinsic fluorescence for the differentiation of lung neoplasms, inflammatory and tumor-free lung, which motivates the application of multiphoton endoscopy for the in situ imaging of lung nodules.
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Registered trials
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