ArticleBiophysical journal2012
Label-free imaging of lipid-droplet intracellular motion in early Drosophila embryos using femtosecond-stimulated Raman loss microscopy.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 65 citations in OpenAlex.
- Longitudinal live-cell chemical imaging using coherent Raman scattering microscopy.The Analyst · 2026Review
- Chem-SIM: SIM-resolved chemical imaging via wide-field mid-infrared photothermal modulation of fluorescence.Nature communications · 2026Article
- On the importance of simultaneous label-free multimodal nonlinear optical imaging for biomedical applications.APL photonics · 2025Article
- Highly dynamic mechanical transitions in embryonic cell populations during Drosophila gastrulation.Nature communications · 2025Article
- Principle of Stimulated Raman Scattering Microscopy: Emerging at High Spatiotemporal Limits.The journal of physical chemistry. C, Nanomaterials and interfaces · 2025Article
- How it feels in a cell.Trends in cell biology · 2023Review
- Coherent Raman scattering microscopy of lipid droplets in cells and tissues.Journal of Raman spectroscopy : JRS · 2023Article
- Label-free structural and functional volumetric imaging by dual-modality optical-Raman projection tomography.Science advances · 2023Article
- Stimulated Raman Scattering Imaging Sheds New Light on Lipid Droplet Biology.The journal of physical chemistry. B · 2023Article
- ARP2/3 Regulates Fatty Acid Synthesis by Modulating Lipid Droplets' Motility.International journal of molecular sciences · 2022Article
- Dissecting lipid droplet biology with coherent Raman scattering microscopy.Journal of cell science · 2022Review
- Microtubule motor driven interactions of lipid droplets: Specificities and opportunities.Frontiers in cell and developmental biology · 2022Review
- Visualizing Cytoskeleton-Dependent Trafficking of Lipid-Containing Organelles in Drosophila Embryos.Journal of visualized experiments : JoVE · 2021Article
- Microsecond fingerprint stimulated Raman spectroscopic imaging by ultrafast tuning and spatial-spectral learning.Nature communications · 2021Article
- Motility Plays an Important Role in the Lifetime of Mammalian Lipid Droplets.International journal of molecular sciences · 2021Review
- Tracking the formation and degradation of fatty-acid-accumulated mitochondria using label-free chemical imaging.Scientific reports · 2021Article
- Label-free optical imaging in developmental biology [Invited].Biomedical optics express · 2020Review
- Three-dimensional label-free imaging throughout adipocyte differentiation by stimulated Raman microscopy.PloS one · 2019Article
- Deciphering single cell metabolism by coherent Raman scattering microscopy.Current opinion in chemical biology · 2016Review
- In Situ and In Vivo Molecular Analysis by Coherent Raman Scattering Microscopy.Annual review of analytical chemistry (Palo Alto, Calif.) · 2016Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Lipid droplets are complex organelles that exhibit highly dynamic behavior in early Drosophila embryo development. Imaging lipid droplet motion provides a robust platform for the investigation of shuttling by kinesin and dynein motors, but methods for imaging are either destructive or deficient in resolution and penetration to study large populations of droplets in an individual embryo. Here we report real-time imaging and quantification of droplet motion in live embryos using a recently developed technique termed "femtosecond-stimulated Raman loss" microscopy. We captured long-duration time-lapse images of the developing embryo, tracked single droplet motion within large populations of droplets, and measured the velocity and turning frequency of each particle at different apical-to-basal depths and stages of development. To determine whether the quantities for speed and turning rate measured for individual droplets are sufficient to predict the population distributions of droplet density, we simulated droplet motion using a velocity-jump model. This model yielded droplet density distributions that agreed well with experimental observations without any model optimization or unknown parameter estimation, demonstrating the sufficiency of a velocity-jump process for droplet trafficking dynamics in blastoderm embryos.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.