ArticleBiophysical journal2012
End-to-end self-assembly of RADA 16-I nanofibrils in aqueous solutions.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed.
- First Safety and Performance Evaluation of T45K, a Self-Assembling Peptide Barrier Hemostatic Device, After Skin Lesion Excision.Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.] · 2018Trial
- RADA16 as a novel hemostatic and regenerative agent in urology: European Association of Urology endourology up-to-date overview.Current opinion in urology · 2026Review
- A Photoresponsive Hybrid of Viruses and Supramolecular Peptide Fibers for Multidimensional Control of Patterning and Infection.Angewandte Chemie (International ed. in English) · 2025Article
- MOTS-c-modified functional self-assembly peptide hydrogels enhance the activity of nucleus pulposus-derived mesenchymal stem cells of intervertebral disc degeneration.Materials today. Bio · 2025Article
- Self-assembling peptides for sciatic nerve regeneration: a review of conduit microenvironment modeling strategies in preclinical studies.Frontiers in cell and developmental biology · 2025Review
- Innovative hydrogels in cutaneous wound healing: current status and future perspectives.Frontiers in bioengineering and biotechnology · 2025Review
- Macrocyclic Peptides Closed by a Thioether-Bipyridyl Unit That Grants Cell Membrane Permeability.ACS bio & med chem Au · 2023Article
- Peptide-Based Hydrogels: Template Materials for Tissue Engineering.Journal of functional biomaterials · 2023Review
- Release systems based on self-assembling RADA16-I hydrogels with a signal sequence which improves wound healing processes.Scientific reports · 2023Article
- Polymeric Hydrogels for In Vitro 3D Ovarian Cancer Modeling.International journal of molecular sciences · 2022Review
- Self-Assembling Peptides: From Design to Biomedical Applications.International journal of molecular sciences · 2021Review
- Straightforward and Cost-Effective Production of RADA-16I Peptide inIranian journal of biotechnology · 2019Article
- Self-assembly behaviours of peptide-drug conjugates: influence of multiple factors on aggregate morphology and potential self-assembly mechanism.Royal Society open science · 2018Article
- RNA extraction from self-assembling peptide hydrogels to allow qPCR analysis of encapsulated cells.PloS one · 2018Article
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- On the lag phase in amyloid fibril formation.Physical chemistry chemical physics : PCCP · 2015Review
- Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RADARADARADARADA (RADA 16-I) is a synthetic amphiphilic peptide designed to self-assemble in a controlled way into fibrils and higher ordered structures depending on pH. In this work, we use various techniques to investigate the state of the peptide dispersed in water under dilute conditions at different pH and in the presence of trifluoroacetic acid or hydrochloric acid. We have identified stable RADA 16-I fibrils at pH 2.0-4.5, which have a length of ∼200-400 nm and diameter of 10 nm. The fibrils have the characteristic antiparallel β-sheet structure of amyloid fibrils, as measured by circular dichroism and Fourier transform infrared spectrometry. During incubation at pH 2.0-4.5, the fibrils elongate very slowly via an end-to-end fibril-fibril aggregation mechanism, without changing their diameter, and the kinetics of such aggregation depends on pH and anion type. At pH 2.0, we also observed a substantial amount of monomers in the system, which do not participate in the fibril elongation and degrade to fragments. The fibril-fibril elongation kinetics has been simulated using the Smoluchowski kinetic model, population balance equations, and the simulation results are in good agreement with the experimental data. It is also found that the aggregation process is not limited by diffusion but rather is an activated process with energy barrier in the order of 20 kcal/mol.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.