ArticleBiophysical journal2012
Super-resolution imaging of C-type lectin and influenza hemagglutinin nanodomains on plasma membranes using blink microscopy.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 42 citations in OpenAlex.
- The bat influenza A virus subtype H18N11 induces nanoscale MHCII clustering upon host cell attachment.Nature communications · 2025Article
- Review
- Dectin-1-Mediated DC-SIGN Recruitment toLife (Basel, Switzerland) · 2021Article
- Towards a Quantitative Single Particle Characterization by Super Resolution Microscopy: From Virus Structures to Antivirals Design.Frontiers in bioengineering and biotechnology · 2021Review
- Review
- Super-resolution microscopy reveals significant impact of M2e-specific monoclonal antibodies on influenza A virus filament formation at the host cell surface.Scientific reports · 2019Article
- Mannan Molecular Substructures Control Nanoscale Glucan Exposure in Candida.Cell reports · 2018Article
- Rapid, directed transport of DC-SIGN clusters in the plasma membrane.Science advances · 2017Article
- Super-Resolution Microscopy: Shedding Light on the Cellular Plasma Membrane.Chemical reviews · 2017Review
- Beyond attachment: Roles of DC-SIGN in dengue virus infection.Traffic (Copenhagen, Denmark) · 2017Article
- Dynamics of the actin cytoskeleton mediates receptor cross talk: An emerging concept in tuning receptor signaling.The Journal of cell biology · 2016Review
- Hemagglutinin Spatial Distribution Shifts in Response to Cholesterol in the Influenza Viral Envelope.Biophysical journal · 2015Article
- Chemical Lectinology: Tools for Probing the Ligands and Dynamics of Mammalian Lectins In Vivo.Chemistry & biology · 2015Review
- Nanoclustering as a dominant feature of plasma membrane organization.Journal of cell science · 2014Review
- Optimal structural design of mannosylated nanocarriers for macrophage targeting.Journal of controlled release : official journal of the Controlled Release Society · 2014Article
- Super-resolution imaging of C-type lectin spatial rearrangement within the dendritic cell plasma membrane at fungal microbe contact sites.Frontiers in physics · 2014Article
- Enhanced receptor-clathrin interactions induced by N-glycan-mediated membrane micropatterning.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- A new tool to quantify receptor recruitment to cell contact sites during host-pathogen interaction.PLoS computational biology · 2014Article
- Low copy numbers of DC-SIGN in cell membrane microdomains: implications for structure and function.Traffic (Copenhagen, Denmark) · 2014Article
- Super-resolution molecular and functional imaging of nanoscale architectures in life and materials science.Frontiers in bioengineering and biotechnology · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Dendritic cells express DC-SIGN, a C-type lectin (CTL) that binds a variety of pathogens and facilitates their uptake for subsequent antigen presentation. DC-SIGN forms remarkably stable microdomains on the plasma membrane. However, inner leaflet lipid markers are able to diffuse through these microdomains suggesting that, rather than being densely packed with DC-SIGN proteins, an elemental substructure exists. Therefore, a super-resolution imaging technique, Blink Microscopy (Blink), was applied to further investigate the lateral distribution of DC-SIGN. Blink indicates that DC-SIGN, another CTL (CD206), and influenza hemagglutinin (HA) are all localized in small (∼80 nm in diameter) nanodomains. DC-SIGN and CD206 nanodomains are randomly distributed on the plasma membrane, whereas HA nanodomains cluster on length scales up to several microns. We estimate, as a lower limit, that DC-SIGN and HA nanodomains contain on average two tetramers or two trimers, respectively, whereas CD206 is often nonoligomerized. Two-color Blink determined that different CTLs rarely occupy the same nanodomain, although they appear colocalized using wide-field microscopy. What to our knowledge is a novel domain structure emerges in which elemental nanodomains, potentially capable of binding viruses, are organized in a random fashion; evidently, these nanodomains can be clustered into larger microdomains that act as receptor platforms for larger pathogens like yeasts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.