ArticleBiophysical journal2012
Membrane dynamics correlate with formation of signaling clusters during cell spreading.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Exploring heterogeneous cell population dynamics in different microenvironments by novel analytical strategy based on images.NPJ systems biology and applications · 2024Article
- Nanotopography modulates cytoskeletal organization and dynamics during T cell activation.Molecular biology of the cell · 2022Article
- Cytotoxic T Lymphocyte Activation Signals Modulate Cytoskeletal Dynamics and Mechanical Force Generation.Frontiers in immunology · 2022Article
- WASp Is Crucial for the Unique Architecture of the Immunological Synapse in Germinal Center B-Cells.Frontiers in cell and developmental biology · 2021Article
- High-performance imaging of cell-substrate contacts using refractive index quantification microscopy.Biomedical optics express · 2020Article
- Bcl10 is associated with actin dynamics at the T cell immune synapse.Cellular immunology · 2020Article
- Correlative three-dimensional super-resolution and block-face electron microscopy of whole vitreously frozen cells.Science (New York, N.Y.) · 2020Article
- Recent Advances and Prospects in the Research of Nascent Adhesions.Frontiers in physiology · 2020Review
- Mechanosensing in the immune response.Seminars in cell & developmental biology · 2017Review
- Dynamic microtubules regulate cellular contractility during T-cell activation.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Article
- Existence of and decay to equilibrium of the filament end density along the leading edge of the lamellipodium.Journal of mathematical biology · 2017Article
- Frustrated Phagocytic Spreading of J774A-1 Macrophages Ends in Myosin II-Dependent Contraction.Biophysical journal · 2016Article
- Cytoskeletal forces during signaling activation in Jurkat T-cells.Molecular biology of the cell · 2015Article
- A defensin fragment regulates CHO-K1 cell spreading and induces renewal of fatty acid composition in cell membrane phospholipids.Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections · 2015Article
- Ligand-mediated friction determines morphodynamics of spreading T cells.Biophysical journal · 2014Article
- Automatic sorting of point pattern sets using Minkowski functionals.Physical review. E, Statistical, nonlinear, and soft matter physics · 2013Article
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The morphology and duration of contacts between cells and adhesive surfaces play a key role in several biological processes, such as cell migration, cell differentiation, and the immune response. The interaction of receptors on the cell membrane with ligands on the adhesive surface leads to triggering of signaling pathways, which allow cytoskeletal rearrangement, and large-scale deformation of the cell membrane, which allows the cell to spread over the substrate. Despite numerous studies of cell spreading, the nanometer-scale dynamics of the membrane during formation of contacts, spreading, and initiation of signaling are not well understood. Using interference reflection microscopy, we study the kinetics of cell spreading at the micron scale, as well as the topography and fluctuations of the membrane at the nanometer scale during spreading of Jurkat T cells on antibody-coated substrates. We observed two modes of spreading, which were characterized by dramatic differences in membrane dynamics and topography. Formation of signaling clusters was closely related to the movement and morphology of the membrane in contact with the activating surface. Our results suggest that cell membrane morphology may be a critical constraint on signaling at the cell-substrate interface.
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