ArticleTranslational research : the journal of laboratory and clinical medicine2012
Molecular profiling of individual tumor cells by hyperspectral microscopic imaging.
Article in Translational research : the journal of laboratory and clinical medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.
- Hyperspectral imaging for tumor resection guidance in surgery: a systematic review of preclinical and clinical studies.Journal of biomedical optics · 2025Pooled it
- In vitro hyperspectral biomarkers of human chondrosarcoma cells in nanoparticle-mediated radiosensitization using carbon ions.Scientific reports · 2023Article
- Classification of two species of Gram-positive bacteria through hyperspectral microscopy coupled with machine learning.Biomedical optics express · 2021Article
- Hyperspectral Imagery for Assessing Laser-Induced Thermal State Change in Liver.Sensors (Basel, Switzerland) · 2021Article
- Immunomagnetic Capture and Multiplexed Surface Marker Detection of Circulating Tumor Cells with Magnetic Multicolor Surface-Enhanced Raman Scattering Nanotags.ACS applied materials & interfaces · 2020Article
- Feasibility of hyperspectral analysis for discrimination of rabbit liver VX2 tumor.World journal of gastrointestinal oncology · 2019Article
- A Novel Hyperspectral Microscopic Imaging System for Evaluating Fresh Degree of Pork.Korean journal for food science of animal resources · 2018Article
- Article
- Optical hyperspectral imaging in microscopy and spectroscopy - a review of data acquisition.Journal of biophotonics · 2015Review
- Molecular heterogeneity in adjacent cells in triple-negative breast cancer.Breast cancer (Dove Medical Press) · 2015Article
- Use of colloidal quantum dots as a digitally switched swept light source for gold nanoparticle based hyperspectral microscopy.Biomedical optics express · 2014Article
- Medical hyperspectral imaging: a review.Journal of biomedical optics · 2014Review
- Single cell spectroscopy: noninvasive measures of small-scale structure and function.Methods (San Diego, Calif.) · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 4 institutions in 1 country.
Funding
Abstract
We developed a hyperspectral microscopic imaging (HMI) platform that can precisely identify and quantify 10 molecular markers in individual cancer cells in a single pass. The exploitation of an improved separation of circulating tumor cells and the application of HMI provided an opportunity (1) to identify molecular changes in these cells, (2) to recognize the coexpression of these markers, (3) to pose an important opportunity for noninvasive diagnosis, and (4) to use targeted therapy. We balanced the intensity of 10 fluorochromes bound to 10 different antibodies, each specific to a particular tumor marker, so that the intensity of each fluorochrome can be discerned from overlapping emissions. Using 2 touch preps from each primary breast cancer, the average molecular marker intensities of 25 tumor cells gave a representative molecular signature for the tumor despite some cellular heterogeneity. The intensities determined by the HMI correlate well with the conventional 0-3+ analysis by experts in cellular pathology. Because additional multiplexes can be developed using the same fluorochromes but different antibodies, this analysis allows quantification of many molecular markers on a population of tumor cells. HMI can be automated completely, and eventually, it could allow the standardization of protein biomarkers and improve reproducibility among clinical pathology laboratories.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.