ArticleJournal of amino acids2012
Real-Time Analysis of Specific Protein-DNA Interactions with Surface Plasmon Resonance.
Article in Journal of amino acids, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Surface Plasmon Resonance Imaging and Microscopy Modalities for Information-Rich, Label-Free Analysis of Biomolecular Interactions and Disease Biomarkers.Chemical & biomedical imaging · 2026Review
- The emerging role of aptamers in targeted cancer immunotherapy.Molecular therapy. Oncology · 2026Review
- Surface Plasmon Resonance for the Interaction of Capsular Polysaccharide (CPS) With KpACE.Bio-protocol · 2025Article
- The potential of a novel enzyme-based surface plasmon resonance biosensor for direct detection of dopamine.Scientific reports · 2024Article
- Unraveling the Dynamics of SARS-CoV-2 Mutations: Insights from Surface Plasmon Resonance Biosensor Kinetics.Biosensors · 2024Review
- Structural basis of DNA binding by the NAC transcription factor ORE1, a master regulator of plant senescence.Plant communications · 2023Article
- Insight into the biochemical mechanism of DNA helicases provided by bulk-phase and single-molecule assays.Methods (San Diego, Calif.) · 2022Review
- Exosome Processing and Characterization Approaches for Research and Technology Development.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Tuning and Sensitivity Improvement of Bi-Metallic Structure-Based Surface Plasmon Resonance Biosensor with 2-DPlasmonics (Norwell, Mass.) · 2022Article
- Toward G-Quadruplex-Based Anticancer Agents: Biophysical and Biological Studies of Novel AS1411 Derivatives.International journal of molecular sciences · 2020Article
- Exploring Graphene and MoSFrontiers in chemistry · 2020Review
- Discovery of novel elongator protein 2 inhibitors by compound library screening using surface plasmon resonance.RSC advances · 2019Article
- Development of a bioaffinity SPR immunosensor based on functionalized graphene oxide for the detection of pregnancy-associated plasma protein A2 in human plasma.International journal of nanomedicine · 2019Article
- Chitosan in Non-Viral Gene Delivery: Role of Structure, Characterization Methods, and Insights in Cancer and Rare Diseases Therapies.Polymers · 2018Review
- Measuring affinity constants of 1450 monoclonal antibodies to peptide targets with a microarray-based label-free assay platform.Journal of immunological methods · 2015Article
- Real-time surface plasmon resonance study of biomolecular interactions between polymerase and bulky mutagenic DNA lesions.Chemical research in toxicology · 2014Article
- Surface plasmon resonance for cell-based clinical diagnosis.Sensors (Basel, Switzerland) · 2014Review
- Structure elucidation of DNA-protein crosslinks by using reductive desulfurization and liquid chromatography-tandem mass spectrometry.Chembiochem : a European journal of chemical biology · 2014Article
- Synthesis of sequence-specific DNA-protein conjugates via a reductive amination strategy.Bioconjugate chemistry · 2013Article
- Binary and ternary binding affinities between exonuclease-deficient Klenow fragment (Kf-exo(-)) and various arylamine DNA lesions characterized by surface plasmon resonance.Chemical research in toxicology · 2012Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several proteins, like transcription factors, bind to certain DNA sequences, thereby regulating biochemical pathways that determine the fate of the corresponding cell. Due to these key positions, it is indispensable to analyze protein-DNA interactions and to identify their mode of action. Surface plasmon resonance is a label-free method that facilitates the elucidation of real-time kinetics of biomolecular interactions. In this article, we focus on this biosensor-based method and provide a detailed guide how SPR can be utilized to study binding of proteins to oligonucleotides. After a description of the physical phenomenon and the instrumental realization including fiber-optic-based SPR and SPR imaging, we will continue with a survey of immobilization methods. Subsequently, we will focus on the optimization of the experiment, expose pitfalls, and introduce how data should be analyzed and published. Finally, we summarize several interesting publications of the last decades dealing with protein-DNA and RNA interaction analysis by SPR.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.