ArticleThe Journal of biological chemistry2012
Borrelia burgdorferi, a pathogen that lacks iron, encodes manganese-dependent superoxide dismutase essential for resistance to streptonigrin.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 64 citations in OpenAlex.
- Actinobacillus seminis DnaK interacts with bovine transferrin, lactoferrin, and hemoglobin as a putative iron acquisition mechanism.Folia microbiologica · 2026Article
- Impact of Iron Deficiency on the Growth and Bioelectrical Profile of Different Gut Bacteria.MicrobiologyOpen · 2025Article
- Article
- Heme and iron limitation in a GI-tract foundation species leads to a reshuffling of the metalloproteome and a shift toward manganese usage.Frontiers in chemistry · 2025Article
- CspZ variant-specific interaction with factor H incorporates a metal site to support Lyme borreliae complement evasion.The Journal of biological chemistry · 2025Article
- Assessment of the hypothetical protein BB0616 in the murine infection ofInfection and immunity · 2024Article
- Can iron chelators ameliorate viral infections?Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2024Review
- Modulation of Macrophage Redox and Apoptotic Processes toPathogens (Basel, Switzerland) · 2023Article
- Immune evasion strategies of major tick-transmitted bacterial pathogens.Trends in microbiology · 2023Review
- Large-Scale Sequencing ofGenes · 2022Review
- Old dogs, new tricks: New insights into the iron/manganese superoxide dismutase family.Journal of inorganic biochemistry · 2022Article
- The Brilliance ofPathogens (Basel, Switzerland) · 2021Review
- Lyme Disease Pathogenesis.Current issues in molecular biology · 2021Review
- Siderophore-Mediated Iron Acquisition Plays a Critical Role in Biofilm Formation and Survival ofFrontiers in medicine · 2021Article
- FightingFrontiers in cellular and infection microbiology · 2021Review
- Unconventional Approaches to Direct Detection of Borreliosis and Other Tick Borne Illnesses: A Path Forward.Journal of cellular immunology · 2021Article
- AttenuatedVaccines · 2020Article
- A Siderophore Analog of Fimsbactin fromPathogens (Basel, Switzerland) · 2020Article
- Establishment of an in vitro RNA polymerase transcription system: a new tool to study transcriptional activation in Borrelia burgdorferi.Scientific reports · 2020Article
- The Lyme disease spirochete's BpuR DNA/RNA-binding protein is differentially expressed during the mammal-tick infectious cycle, which affects translation of the SodA superoxide dismutase.Molecular microbiology · 2019Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Borrelia burgdorferi, the causative agent of Lyme disease, exists in nature through a complex life cycle involving ticks of the Ixodes genus and mammalian hosts. During its life cycle, B. burgdorferi experiences fluctuations in oxygen tension and may encounter reactive oxygen species (ROS). The key metalloenzyme to degrade ROS in B. burgdorferi is SodA. Although previous work suggests that B. burgdorferi SodA is an iron-dependent superoxide dismutase (SOD), later work demonstrates that B. burgdorferi is unable to transport iron and contains an extremely low intracellular concentration of iron. Consequently, the metal cofactor for SodA has been postulated to be manganese. However, experimental evidence to support this hypothesis remains lacking. In this study, we provide biochemical and genetic data showing that SodA is a manganese-dependent enzyme. First, B. burgdorferi contained SOD activity that is resistant to H(2)O(2) and NaCN, characteristics associated with Mn-SODs. Second, the addition of manganese to the Chelex-treated BSK-II enhanced SodA expression. Third, disruption of the manganese transporter gene bmtA, which significantly lowers the intracellular manganese, greatly reduced SOD activity and SodA expression, suggesting that manganese regulates the level of SodA. In addition, we show that B. burgdorferi is resistant to streptonigrin, a metal-dependent redox cycling compound that produces ROS, and that SodA plays a protective role against the streptonigrin. Taken together, our data demonstrate the Lyme disease spirochete encodes a manganese-dependent SOD that contributes to B. burgdorferi defense against intracellular superoxide.
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Registered trials
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