ArticleJournal of applied physiology (Bethesda, Md. : 1985)2012
Endothelial nitric oxide synthase mediates cutaneous vasodilation during local heating and is attenuated in middle-aged human skin.
Article in Journal of applied physiology (Bethesda, Md. : 1985), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
68 citing papers in PubMed, 120 citations in OpenAlex.
- Acute effects of hypouricemia on endothelium, oxidative stress, and arterial stiffness: A randomized, double-blind, crossover study.Physiological reports · 2021Trial
- ETAmerican journal of physiology. Heart and circulatory physiology · 2021Trial
- Four weeks of vitamin D supplementation improves nitric oxide-mediated microvascular function in college-aged African Americans.American journal of physiology. Heart and circulatory physiology · 2020Trial
- Controlled Feeding of an 8-d, High-Dairy Cheese Diet Prevents Sodium-Induced Endothelial Dysfunction in the Cutaneous Microcirculation of Healthy, Older Adults through Reductions in Superoxide.The Journal of nutrition · 2020Trial
- Apocynin and Tempol ameliorate dietary sodium-induced declines in cutaneous microvascular function in salt-resistant humans.American journal of physiology. Heart and circulatory physiology · 2019Trial
- Effects of aerobic exercise on vascular function in nondialysis chronic kidney disease: a randomized controlled trial.American journal of physiology. Renal physiology · 2019Trial
- Effects of vehicle microdialysis solutions on cutaneous vascular responses to local heating.Journal of applied physiology (Bethesda, Md. : 1985) · 2017Trial
- Folic acid supplementation improves microvascular function in older adults through nitric oxide-dependent mechanisms.Clinical science (London, England : 1979) · 2015Trial
- Dietary sodium loading impairs microvascular function independent of blood pressure in humans: role of oxidative stress.The Journal of physiology · 2012Trial
- Thermal-enhanced self-heating microneedle patch for sustained progesterone delivery and improved pregnancy maintenance.Materials today. Bio · 2026Article
- Cutaneous microvascular function is reduced in winter compared with summer in healthy young adults.Physiological reports · 2026Article
- Short-term estradiol administration does not restore endothelin-B receptor-mediated vasodilation in postmenopausal women.American journal of physiology. Heart and circulatory physiology · 2025Article
- Quantification and interpretation of nitric oxide-dependent cutaneous vasodilation during local heating.Journal of applied physiology (Bethesda, Md. : 1985) · 2024Article
- Sex differences in heat stress vulnerability among middle-aged and older adults (PSU HEAT Project).American journal of physiology. Regulatory, integrative and comparative physiology · 2024Article
- Inhibition of superoxide and iNOS augment cutaneous nitric oxide-dependent vasodilation in non-Hispanic black young adults.Physiological reports · 2024Article
- Impaired microvascular reactivity in patients treated with 5-fluorouracil chemotherapy regimens: Potential role of endothelial dysfunction.International journal of cardiology. Heart & vasculature · 2023Article
- Angiotensin II type 2 receptor-mediated dilation is greater in the cutaneous microvasculature of premenopausal women compared with men.Journal of applied physiology (Bethesda, Md. : 1985) · 2023Article
- Within-limb variation in skin pigmentation does not influence cutaneous vasodilation.Journal of applied physiology (Bethesda, Md. : 1985) · 2023Article
- Combining hypoxia with thermal stimuli in humans: physiological responses and potential sex differences.American journal of physiology. Regulatory, integrative and comparative physiology · 2023Review
- Effects of biological sex and oral contraceptive pill use on cutaneous microvascular endothelial function and nitric oxide-dependent vasodilation in humans.Journal of applied physiology (Bethesda, Md. : 1985) · 2023Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Local skin heating is used to assess microvascular function in clinical populations because NO is required for full expression of the response; however, controversy exists as to the precise NO synthase (NOS) isoform producing NO. Human aging is associated with attenuated cutaneous vasodilation but little is known about the middle aged, an age cohort used for comparison with clinical populations. We hypothesized that endothelial NOS (eNOS) is the primary isoform mediating NO production during local heating, and eNOS-dependent vasodilation would be reduced in middle-aged skin. Vasodilation was induced by local heating (42°C) and during acetylcholine dose-response (ACh-DR: 0.01, 0.1, 1.0, 5.0, 10.0, 50.0, 100.0 mmol/l) protocols. Four microdialysis fibers were placed in the skin of 24 men and women; age cohorts were 12 middle-aged (53 ± 1 yr) and 12 young (23 ± 1 yr). Sites served as control, nonselective NOS inhibited [N(G)-nitro-l-arginine methyl ester (l-NAME)], inducible NOS (iNOS) inhibited (1400W), and neuronal NOS (nNOS) inhibited (N(ω)-propyl-l-arginine). After full expression of the local heating response, l-NAME was perfused at all sites. Cutaneous vascular conductance was measured and normalized to maximum (%CVC(max): Nitropress). l-NAME reduced %CVCmax at baseline, all phases of the local heating response, and at all ACh concentrations compared with all other sites. iNOS inhibition reduced the initial peak (53 ± 2 vs. 60 ± 2%CVC(max); P < 0.001); however, there were no other differences between control, nNOS-, and iNOS-inhibited sites during the phases of local heating or ACh-DR. When age cohorts were compared, NO-dependent vasodilation during local heating (52 ± 6 vs. 68 ± 4%CVC(max); P = 0.013) and ACh perfusion (50 mmol/l: 83 ± 3 vs. 93 ± 2%CVC(max); 100 mmol/l: 83 ± 4 vs. 92 ± 3%CVC(max); both P = 0.03) were reduced in middle-aged skin. There were no differences in NOS isoform expression obtained from skin biopsy samples between groups (all P > 0.05). These data suggest that eNOS mediates the production of NO during local heating and that cutaneous vasodilation is attenuated in middle-aged skin.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.