ArticleArteriosclerosis, thrombosis, and vascular biology2012
A leukocyte-mimetic magnetic resonance imaging contrast agent homes rapidly to activated endothelium and tracks with atherosclerotic lesion macrophage content.
Article in Arteriosclerosis, thrombosis, and vascular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 57 citations in OpenAlex.
- Translational Molecular Imaging Tool of Vulnerable Carotid Plaque: Evaluate Effects of Statin Therapy on Plaque Inflammation and American Heart Association-Defined Risk Levels in Cuff-Implanted Apolipoprotein E-Deficient Mice.Translational stroke research · 2024Article
- Development of Molecular Magnetic Resonance Imaging Tools for Longitudinal Tracking of Carotid Atherosclerotic Disease Using Fast Imaging with Steady-State Precession.Translational stroke research · 2023Article
- MRI Contrast Agents in Glycobiology.Molecules (Basel, Switzerland) · 2022Review
- Development of Molecular Magnetic Resonance Imaging Tools for Risk Stratification of Carotid Atherosclerotic Disease Using Dual-Targeted Microparticles of Iron Oxide.Translational stroke research · 2022Article
- Targeting pulmonary vascular endothelial cells for the treatment of respiratory diseases.Frontiers in pharmacology · 2022Review
- Improved detection of molecularly targeted iron oxide particles in mouse brain using BMagnetic resonance imaging · 2020Article
- Molecular Imaging of a New Multimodal Microbubble for Adhesion Molecule Targeting.Cellular and molecular bioengineering · 2019Article
- Chemistry of MRI Contrast Agents: Current Challenges and New Frontiers.Chemical reviews · 2019Review
- Targeted Transfection Using PEGylated Cationic Liposomes Directed Towards P-Selectin Increases siRNA Delivery into Activated Endothelial Cells.Pharmaceutics · 2019Article
- Monocyte and Macrophage Dynamics in the Cardiovascular System: JACC Macrophage in CVD Series (Part 3).Journal of the American College of Cardiology · 2018Review
- Optimization of molecularly targeted MRI in the brain: empirical comparison of sequences and particles.International journal of nanomedicine · 2018Article
- MRI detection of endothelial cell inflammation using targeted superparamagnetic particles of iron oxide (SPIO).Clinical and translational medicine · 2017Article
- Inflammatory processes in cardiovascular disease: a route to targeted therapies.Nature reviews. Cardiology · 2017Review
- Advances in Monitoring Cell-Based Therapies with Magnetic Resonance Imaging: Future Perspectives.International journal of molecular sciences · 2017Review
- Molecular magnetic resonance imaging discloses endothelial activation after transient ischaemic attack.Brain : a journal of neurology · 2017Article
- Drug carrier interaction with blood: a critical aspect for high-efficient vascular-targeted drug delivery systems.Therapeutic delivery · 2015Review
- Molecular magnetic resonance imaging of angiogenesis in vivo using polyvalent cyclic RGD-iron oxide microparticle conjugates.Theranostics · 2015Article
- Combination-targeting to multiple endothelial cell adhesion molecules modulates binding, endocytosis, and in vivo biodistribution of drug nanocarriers and their therapeutic cargoes.Journal of controlled release : official journal of the Controlled Release Society · 2014Article
- Inflammation and neovascularization intertwined in atherosclerosis: imaging of structural and molecular imaging targets.Circulation · 2014Review
- Imaging macrophages with nanoparticles.Nature materials · 2014Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
objectiveEndothelial cell activation is an important mediator of monocyte recruitment to sites of vascular inflammation. We hypothesized that high-affinity dual-ligand microparticles of iron oxide (MPIO), targeted to P-selectin and vascular cell adhesion molecule-1 (PV-MPIO), would identify activated endothelial cells during atherosclerosis progression. METHODS AND
resultsIn vivo magnetic resonance imaging in apolipoprotein E-deficient mice showed rapid binding of PV-MPIO to the aortic root, which was maximal 30 minutes post-MPIO injection and maintained at 60 minutes. Minimal binding was observed for control IgG-MPIO. Intensely low magnetic resonance signal areas, corresponding to PV-MPIO binding, were detected early (14 weeks), during foam cell formation. Contrast effects increased at 20 weeks during fibrofatty lesion development (P<0.05), but reduced by 30 weeks (P<0.01). Across all lesion severities, magnetic resonance imaging contrast effects correlated with lesion macrophage area quantified by immunohistochemistry (R=0.53; P<0.01). Near-infrared fluorescently labeled PV-MPIO were shown, by flow cytometry, to bind only activated endothelial cells and not to macrophages. Using en face immunofluorescence, we further demonstrate selective PV-MPIO accumulation at atherosclerosis-susceptible sites, with minimal binding to atherosclerosis-spared regions.
conclusionsThis high-affinity leukocyte-mimetic magnetic resonance imaging agent reveals endothelial activation. PV-MPIO demonstrate exceptionally rapid in vivo steady state accumulation, providing conspicuous magnetic resonance contrast effects that can be objectively quantified. In atherosclerosis progression, PV-MPIO tracked closely with the burden and distribution of plaque macrophages, not merely plaque size. On a biocompatible platform, this approach has potential for quantitative magnetic resonance imaging of inflammatory disease activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.