ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2012
A Gal4/UAS system for conditional transgene expression in rhombomere 4 of the zebrafish hindbrain.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 10 citations in OpenAlex.
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- Celsr3 drives development and connectivity of the acoustic startle hindbrain circuit.PLoS genetics · 2024Article
- Celsr3 drives development and connectivity of the acoustic startle hindbrain circuit.bioRxiv : the preprint server for biology · 2024Article
- Presynaptic Inhibition Selectively Gates Auditory Transmission to the Brainstem Startle Circuit.Current biology : CB · 2018Article
- A GAL4-inducible transgenic tool kit for the in vivo modulation of Rho GTPase activity in zebrafish.Developmental dynamics : an official publication of the American Association of Anatomists · 2016Article
- PCP Signaling between Migrating Neurons and their Planar-Polarized Neuroepithelial Environment Controls Filopodial Dynamics and Directional Migration.PLoS genetics · 2016Article
- A controllable on-off strategy for the reproductive containment of fish.Scientific reports · 2015Article
- prdm12b specifies the p1 progenitor domain and reveals a role for V1 interneurons in swim movements.Developmental biology · 2014Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe zebrafish is well established as a model organism for the study of vertebrate embryogenesis, but transgenic lines enabling restricted gene expression are still lacking for many tissues.
resultsWe first generated the hoxb1a(β-globin):eGFP(um8) line that expresses eGFP in hindbrain rhombomere 4 (r4), as well as in facial motor neurons migrating caudally from r4. Second, we generated the hoxb1a(β-globin) Gal4VP16(um60) line to express the exogenous Gal4VP16 transcription factor in r4. Lastly, we prepared the UAS(β-actin):hoxa3a(um61) line where the hoxa3a gene, which is normally expressed in r5 and r6, is under control of Gal4-regulated UAS elements. Crossing the hoxb1a(β-globin):Gal4VP16(um60) line to the UAS(β-actin):hoxa3a(um61) line drives robust hoxa3a expression in r4. We find that transgenic expression of hoxa3a in r4 does not affect hoxb1a expression, but has variable effects on migration of facial motorneurons and formation of Mauthner neurons. While cases of somatic transgene silencing have been reported in zebrafish, we have not observed such silencing to date, possibly because of our efforts to minimize repetitive sequences in the transgenic constructs.
conclusionWe have generated three transgenic lines that will be useful for future studies by permitting the labeling of r4-derived cells, as well as by enabling r4-specific expression of various transgenes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.