ArticlePloS one2012
A BAC-based transgenic mouse specifically expresses an inducible Cre in the urothelium.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 17 citations in OpenAlex.
- PTEN modulates urinary tract infection susceptibility and shapes urothelial antibacterial defenses.Life science alliance · 2025Article
- PPARγ promotes urothelial remodeling during urinary tract obstruction.Experimental & molecular medicine · 2025Article
- Insulin receptor signaling engages bladder urothelial defenses that limit urinary tract infection.Cell reports · 2024Article
- Novel Mouse Models of Bladder Cancer Identify a Prognostic Signature Associated with Risk of Disease Progression.Cancer research · 2021Article
- Functional roles for PIEZO1 and PIEZO2 in urothelial mechanotransduction and lower urinary tract interoception.JCI insight · 2021Article
- Urothelium-Specific Deletion of Connexin43 in the Mouse Urinary Bladder Alters Distension-Induced ATP Release and Voiding Behavior.International journal of molecular sciences · 2021Article
- The Urothelium: Life in a Liquid Environment.Physiological reviews · 2020Review
- Pparg promotes differentiation and regulates mitochondrial gene expression in bladder epithelial cells.Nature communications · 2019Article
- Polyploid Superficial Cells that Maintain the Urothelial Barrier Are Produced via Incomplete Cytokinesis and Endoreplication.Cell reports · 2018Article
- The uroplakin plaque promotes renal structural integrity during congenital and acquired urinary tract obstruction.American journal of physiology. Renal physiology · 2018Article
- Mode of Surgical Injury Influences the Source of Urothelial Progenitors during Bladder Defect Repair.Stem cell reports · 2017Article
- Modelling bladder cancer in mice: opportunities and challenges.Nature reviews. Cancer · 2015Review
- Bladder cancers arise from distinct urothelial sub-populations.Nature cell biology · 2014Article
- Manipulating the Mouse Genome Using Recombineering.Advancements in genetic engineering · 2013Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Cre-loxp mediated conditional knockout strategy has played critical roles for revealing functions of many genes essential for development, as well as the causal relationships between gene mutations and diseases in the postnatal adult mice. One key factor of this strategy is the availability of mice with tissue- or cell type-specific Cre expression. However, the success of the traditional molecular cloning approach to generate mice with tissue specific Cre expression often depends on luck. Here we provide a better alternative by using bacterial artificial chromosome (BAC)-based recombineering to insert iCreERT2 cDNA at the ATG start of the Upk2 gene. The BAC-based transgenic mice express the inducible Cre specifically in the urothelium as demonstrated by mRNA expression and staining for LacZ expression after crossing with a Rosa26 reporter mouse. Taking into consideration the size of the gene of interest and neighboring genes included in a BAC, this method should be widely applicable for generation of mice with tissue specific gene expression or deletions in a more specific manner than previously reported.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.