ArticlePloS one2012
Selection of suitable reference genes for RT-qPCR analyses in cyanobacteria.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed.
- Article
- Development of a CRISPR activation system for targeted gene upregulation in Synechocystis sp. PCC 6803.Communications biology · 2025Article
- dCas12a-mediated CRISPR interference for multiplex gene repression in cyanobacteria for enhanced isobutanol and 3-methyl-1-butanol production.Microbial cell factories · 2025Article
- Co-expression of auxiliary genes enhances the activity of a heterologous OBiotechnology for biofuels and bioproducts · 2025Article
- The Dynamics of Symbiodiniaceae and Photosynthetic Bacteria Under High-Temperature Conditions.Microbial ecology · 2025Article
- Article
- A toolbox to engineer the highly productive cyanobacterium Synechococcus sp. PCC 11901.Plant physiology · 2024Article
- Biosecurity measures to control hepatitis E virus on European pig farms.Frontiers in veterinary science · 2024Article
- Using Publicly Available RNA-seq Data for Expression Analysis of Genes of Interest.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Role of natural transformation in the evolution of small cryptic plasmids in Synechocystis sp. PCC 6803.Environmental microbiology reports · 2023Article
- Identification and evaluation of suitable reference genes for RT-qPCR analyses in Trichoderma atroviride under varying light conditions.Fungal biology and biotechnology · 2023Article
- Magnetic Fields as Inducers of Phycobiliprotein Production by Synechococcus elongatus PCC 7942.Current microbiology · 2023Article
- Cyanobacterial Algal Bloom Monitoring: Molecular Methods and Technologies for Freshwater Ecosystems.Microorganisms · 2023Review
- CGL160-mediated recruitment of the coupling factor CF1 is required for efficient thylakoid ATP synthase assembly, photosynthesis, and chloroplast development in Arabidopsis.The Plant cell · 2023Article
- Manipulation of topoisomerase expression inhibits cell division but not growth and reveals a distinctive promoter structure in Synechocystis.Nucleic acids research · 2022Article
- Compensatory Transcriptional Response ofMolecules (Basel, Switzerland) · 2022Article
- Article
- The combined impact of low temperatures and shifting phosphorus availability on the competitive ability of cyanobacteria.Scientific reports · 2022Article
- CRISPRi as a Tool to Repress Multiple Copies of Extracellular Polymeric Substances (EPS)-Related Genes in the CyanobacteriumLife (Basel, Switzerland) · 2021Article
- Transcriptome and metabolome analyses of response of Synechocystis sp. PCC 6803 to methyl viologen.Applied microbiology and biotechnology · 2021Article
4 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cyanobacteria are a group of photosynthetic prokaryotes that have a diverse morphology, minimal nutritional requirements and metabolic plasticity that has made them attractive organisms to use in biotechnological applications. The use of these organisms as cell factories requires the knowledge of their physiology and metabolism at a systems level. For the quantification of gene transcripts real-time quantitative polymerase chain reaction (RT-qPCR) is the standard technique. However, to obtain reliable RT-qPCR results the use and validation of reference genes is mandatory. Towards this goal we have selected and analyzed twelve candidate reference genes from three morphologically distinct cyanobacteria grown under routinely used laboratory conditions. The six genes exhibiting less variation in each organism were evaluated in terms of their expression stability using geNorm, NormFinder and BestKeeper. In addition, the minimum number of reference genes required for normalization was determined. Based on the three algorithms, we provide a list of genes for cyanobacterial RT-qPCR data normalization. To our knowledge, this is the first work on the validation of reference genes for cyanobacteria constituting a valuable starting point for future works.
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