ArticlePloS one2012
Effect of cyclic strain on cardiomyogenic differentiation of rat bone marrow derived mesenchymal stem cells.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 84 citations in OpenAlex.
- Role of Inflammatory Niche and Adult Cardiomyocyte Coculture on Differentiation, Matrix Synthesis, and Secretome Release by Human Bone Marrow Mesenchymal Stem Cells.Applied biochemistry and biotechnology · 2022Article
- Soft-Tissue Material Properties and Mechanogenetics during Cardiovascular Development.Journal of cardiovascular development and disease · 2022Review
- Co-growth of Stem Cells With Target Tissue Culture as an Easy and Effective Method of Directed Differentiation.Frontiers in bioengineering and biotechnology · 2021Article
- Cyclic Strain and Electrical Co-stimulation Improve Neural Differentiation of Marrow-Derived Mesenchymal Stem Cells.Frontiers in cell and developmental biology · 2021Article
- Recent Progress in Engineering Mesenchymal Stem Cell Differentiation.Stem cell reviews and reports · 2020Review
- Biological pacemaker: from biological experiments to computational simulation.Journal of Zhejiang University. Science. B · 2020Review
- A resazurin-based, nondestructive assay for monitoring cell proliferation during a scaffold-based 3D culture process.Regenerative biomaterials · 2020Article
- Biomimetic cardiovascular platforms for in vitro disease modeling and therapeutic validation.Biomaterials · 2019Review
- Shear Stress Promotes Arterial Endothelium-Oriented Differentiation of Mouse-Induced Pluripotent Stem Cells.Stem cells international · 2019Article
- Effect of equiaxial cyclic strain on cardiomyogenic induction in mesenchymal stem cells.Progress in biomaterials · 2018Article
- Pre-Conditioning Stem Cells in a Biomimetic Environment for Enhanced Cardiac Tissue Repair:Cellular and molecular bioengineering · 2018Article
- Mechanical stretch and chronotherapeutic techniques for progenitor cell transplantation and biomaterials.BioMedicine · 2018Article
- Stress phase angle regulates differentiation of human adipose-derived stem cells toward endothelial phenotype.Progress in biomaterials · 2018Article
- Strain and Vibration in Mesenchymal Stem Cells.International journal of biomaterials · 2018Review
- A 3D magnetic tissue stretcher for remote mechanical control of embryonic stem cell differentiation.Nature communications · 2017Article
- Steps toward Maturation of Embryonic Stem Cell-Derived Cardiomyocytes by Defined Physical Signals.Stem cell reports · 2017Article
- Enhanced cardiomyogenic induction of mouse pluripotent cells by cyclic mechanical stretch.Biochemical and biophysical research communications · 2017Article
- Thermosensitive and Highly Flexible Hydrogels Capable of Stimulating Cardiac Differentiation of Cardiosphere-Derived Cells under Static and Dynamic Mechanical Training Conditions.ACS applied materials & interfaces · 2016Article
- Biomimetic microstructure morphology in electrospun fiber mats is critical for maintaining healthy cardiomyocyte phenotype.Cellular and molecular bioengineering · 2016Article
- Electrical and mechanical stimulation of cardiac cells and tissue constructs.Advanced drug delivery reviews · 2016Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mesenchymal stem cells (MSCs) are a potential source of material for the generation of tissue-engineered cardiac grafts because of their ability to transdifferentiate into cardiomyocytes after chemical treatments or co-culture with cardiomyocytes. Cardiomyocytes in the body are subjected to cyclic strain induced by the rhythmic heart beating. Whether cyclic strain could regulate rat bone marrow derived MSC (rBMSC) differentiation into cardiomyocyte-like lineage was investigated in this study. A stretching device was used to generate the cyclic strain for rBMSCs. Cardiomyogenic differentiation was evaluated using quantitative real-time reverse transcription polymerase chain reaction (RT-PCR), immunocytochemistry and western-blotting. The results demonstrated that appropriate cyclic strain treatment alone could induce cardiomyogenic differentiation of rBMSCs, as confirmed by the expression of cardiomyocyte-related markers at both mRNA and protein levels. Furthermore, rBMSCs exposed to the strain stimulation expressed cardiomyocyte-related markers at a higher level than the shear stimulation. In addition, when rBMSCs were exposed to both strain and 5-azacytidine (5-aza), expression levels of cardiomyocyte-related markers significantly increased to a degree suggestive of a synergistic interaction. These results suggest that cyclic strain is an important mechanical stimulus affecting the cardiomyogenic differentiation of rBMSCs. This provides a new avenue for mechanistic studies of stem cell differentiation and a new approach to obtain more committed differentiated cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.