ArticlePloS one2012
Direct observation of strand passage by DNA-topoisomerase and its limited processivity.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Geometry of braided DNA dictates supercoiling partitioning.Nature communications · 2026Article
- Substrate accessibility regulation of human TopIIa decatenation by cohesin.Nature communications · 2025Article
- Modeling multiple duplex DNA attachments in a force-extension experiment.Biophysical reports · 2022Article
- Radiosensitization Effect of Gold Nanoparticles on Plasmid DNA Damage Induced by Therapeutic MV X-rays.Nanomaterials (Basel, Switzerland) · 2022Article
- Unravelling the mechanisms of Type 1A topoisomerases using single-molecule approaches.Nucleic acids research · 2021Review
- Effect of Gold Nanoparticle Radiosensitization on Plasmid DNA Damage Induced by High-Dose-Rate Brachytherapy.International journal of nanomedicine · 2021Article
- Direct observation of DNA overwinding by reverse gyrase.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- A novel chromatin tether domain controls topoisomerase IIα dynamics and mitotic chromosome formation.The Journal of cell biology · 2013Article
- Chiral discrimination and writhe-dependent relaxation mechanism of human topoisomerase IIα.The Journal of biological chemistry · 2013Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Type-II DNA topoisomerases resolve DNA entanglements such as supercoils, knots and catenanes by passing one segment of DNA duplex through a transient enzyme-bridged double-stranded break in another segment. The ATP-dependent passage reaction has previously been demonstrated at the single-molecule level, showing apparent processivity at saturating ATP. Here we directly observed the strand passage by human topoisomerase IIα, after winding a pair of fluorescently stained DNA molecules with optical tweezers for 30 turns into an X-shaped braid. On average 0.51 ± 0.33 µm (11 ± 6 turns) of a braid was unlinked in a burst of reactions taking 8 ± 4 s, the unlinked length being essentially independent of the enzyme concentration between 0.25-37 pM. The time elapsed before the start of processive unlinking decreased with the enzyme concentration, being ~100 s at 3.7 pM. These results are consistent with a scenario where the enzyme binds to one DNA for a period of ~10 s, waiting for multiple diffusional encounters with the other DNA to transport it across the break ~10 times, and then dissociates from the binding site without waiting for the exhaustion of transportable DNA segments.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.